IFAT在丝虫病血清流行病学监测中的应用研究

用马来丝虫成虫冰冻切片抗原作间接荧光抗体试验（ＩＰＡＴ），以１：２０稀释样本检测丝虫抗体的阳性符合率为９７．５０％，假阳性率为２．４４％。在２８２名流动人口中抗体阳性率为１６．３１％（４６／２８２）。自１９８７年以来我省抽样进行血清流行病学调查，人群丝虫抗体水平逐年下降，１９９２年对２１个县市１０７９８人份血样检测，显示基本消灭丝虫病８～１１年后人群抗体平均阳性率为３．１８％，ＧＭＲＴ为２５．５９，已接近非流行区健康人水平（２．４４％）．结果表明丝虫病流行区经海群生防治后的效果是稳定的，ＩＦＡＴ用于现场监测能反映丝虫病流行的实际情况。

APPLICATION OF IFAT IN THE SERO-EPIDEMIOLOGICAL SURVEILLANCE OF FILARIASIS

Indirect immunofluorescent antibody test(IFAT)using frozen section of adultworms of B.malayi as antigen has been carried out for sero-epidemiology surveillance of filaria-sis since 1987. The sensitivity and specificity were 97.50%and 97. 56%respectively. In 282samples of mobile population ,IFAT positive rate was 16.31%(46/282), among them, 4 caseswere found microfilariemial. In field investigation when the blood samples diluted at 1 :20 titerwas considered as positive. The results of 10 798 sera from 21 endemic counties showed IFATpositive rate was 3.18%,GMRT 25.59 after 8-11 years from basical elimination of filariasis in1992. Both have no statistical difference to that of the population from non-endemic areas(2. 44.It is indicated that the control measures of filariasis with diethylcarbamazine is effec-tive in our Province and the IFAT could be useful for the epidemiological investigation andsurveillance of filariasis.