| SLE患者PBMC中IFN-γ和IL-6的表达 |
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| 引用本文： | 付继成,蒋正义,黄玉玲,徐刚. SLE患者PBMC中IFN-γ和IL-6的表达[J]. 武汉大学学报(医学版), 2002, 23(3): 205-207 |
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| 作者姓名： | 付继成 蒋正义 黄玉玲 徐刚 |
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| 作者单位： | 武汉大学人民医院皮肤性病科,武汉,430060 |
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| 基金项目： | 中华医学会皮肤病学分会 1997年度科研基金资助 |
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| 摘 要： | 目的 :在单个细胞水平上研究系统性红斑狼疮 (SLE)患者外周血单个核细胞 (PBMC)的细胞内细胞因子IFN γ和IL 6的表达与疾病发病的关系。方法 :用酶联免疫斑点法 (ELISPOT)检测患者PBMC的细胞内细胞因子IFN γ和IL 6的表达情况。结果 :活动期SLE患者PBMC中IL 6及Th2 Th1 比值与正常人相比显著升高 (P <0 .0 1)。结论 :在SLE患者PBMC中细胞因子IL 6升高 ,表达倾向于向Th2 偏移 ,Th1 Th2 平衡在SLE发病机制中起重要作用。
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| 关 键 词： | 红斑狼疮,系统性 γ干扰素 白介素6 酶联免疫斑点法 |
| 修稿时间： | 2001-09-24 |
Expression of IFN-γ and IL-6 in PBMC of Patients with Systemic Lupus Erythematosus |
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| Fu Jicheng,Jiang Zhengyi,Huang Yuling,et al. Expression of IFN-γ and IL-6 in PBMC of Patients with Systemic Lupus Erythematosus[J]. Medical Journal of Wuhan University, 2002, 23(3): 205-207 |
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| Authors: | Fu Jicheng Jiang Zhengyi Huang Yuling et al |
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| Affiliation: | Fu Jicheng,Jiang Zhengyi,Huang Yuling,et al Department of Dermatology,Renmin Hospital,Wuhan University,Wuhan 430060,China |
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| Abstract: | Objective: To study the expression of IFN γ and IL 6 in the peripheral blood mononuclear cells of systemic lupus erythematosus (SLE) patients with pathogensis at single level. Methods: Peripheral blood mononuclear cells (PBMC) from 20 patients with SLE stimulated for 6 hours with phorbol myristate (PMA) plus ionomycin in the presence of monensin. Cells were stained for intracellular cytokines such as IFN γ and IL 6 by enzyme linked immunospot assay (ELISPOT). Results: The expression of IL 6 and Th 2/Th 1 ratio were significantly increased in patients compared with 10 healthy individuals (P<0.01). Conclusion: The increased expression of IL 6 and a shift toward Th 2 Cytokine profile is observed in the PBMC of SLE patients. Th 1/Th 2 cytokine balance has important implication in the pathogensis. |
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| Keywords: | lupus erythematosus,systemic IFN γ IL 6 enzyme linked immunospot assay |
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