长期服用辛伐他汀对机械通气肺损伤大鼠肺湿／干重比与蛋白含量的影响

目的对长期服用辛伐他汀大鼠进行机械通气造成急性肺损伤,观察大鼠肺组织病理学变化、湿／干重比（W／D）及蛋白含量的变化,探讨长期服用辛伐他汀对机械通气致急性肺损伤、肺水肿及肺泡通透性的影响。方法32只雄性SD大鼠随机分为4组（n=8）：对照组（A组）不做任何处理;机械通气组（B组）行机械通气,潮气量7ml／kg;辛伐他汀组（C组）进行辛伐他汀（10mg·kg～·d^-1）灌胃处理4周;辛伐他汀＋机械通气组（D组）辛伐他汀灌胃4周后行机械通气,潮气量7ml／kg。B组与D组行机械通气4h后处死大鼠,收集肺组织,苏木精一伊红染色光镜下观察病理学改变,测定肺组织W／D比及蛋白含量。结果与A组相比,B组大鼠肺组织损伤明显,W／D与蛋白含量均明显增高（P〈0．01）;C组各指标无明显变化。与B组相比,D组肺组织病理损伤明显减轻,W／D及蛋白含量明显降低（P〈0．01）。结论长期服用辛伐他汀可以明显降低肺血管与肺泡的通透性,减轻机械通气所致的急性肺损伤、肺水肿。

Impact of long-term use of simvastatin on W/D and protein content in ventilator-induced lung injury in rats

Objective To investigate the impact of long - term use of simvastatin on wet - to - dry weight ratio （ W/ D） and protein content in ventilator - induced lung injury in rats. Methods Thirty - two male SD rats were randomly divided into four groups （n = 8 each） ： group A received room air without mechanical ventilation; group B received tidal volume of 7 ml/kg with mechanical ventilation for 4 h; group C ： animals were orally administered with 10 rag/（ kg . d） simvastatin for 4 weeks; group D： animals were orally administered with 10 mg/（kg . d） simvastatin for 4 weeks and then received tidal volume of 7 ml/kg with mechanical ventilation for 4 h. After 4 h of ventilation, the rats were sacrificed and the lung tissue were collected and stored with correct methods. Pathological changes in the lung was observed by mi- croscope. The measured indexes included W/D of lung and the levels of protein content in lung tissue. Results Pathological damage of lung tissue in group B was significanly more severe than in group A. The levels of protein content in lung tissue and W/D in group B were significantly higher than in group A （ P 〈 0.01 ）. Compared with group B, those indexes in group D were significantly decreased （ P 〈 0.01 ）. Conclusion Long - term use of simvastatin can significantly reduce pulmonary vascular and alveolar permeability and lighten pulmonary edema c causedventilation in acute lung injury.