Fluorescence in situ hybridization to determine engraftment status after murine bone marrow transplant. |
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| Authors: | A L Hawkins R J Jones B A Zehnbauer M S Zicha M J Collector S J Sharkis C A Griffin |
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| Affiliation: | Johns Hopkins Oncology Center, Baltimore, MD 21205. |
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| Abstract: | The mouse Y-specific DNA sequence pY2 was used as a probe for fluorescence in situ hybridization (FISH) to evaluate murine hematopoietic tissues after sex-mismatched bone marrow transplant (BMT). The pY2 probe was localized to the long arm of the Y chromosome on BM metaphases. Hybridization of pY2 in FISH of interphase cells from BM, spleen, and thymus after BMT was compared with Southern blot analysis; both methods gave comparable results. Only FISH was able to analyze post-BMT peripheral blood (PB) samples successfully, and provides a useful method for following engraftment status in the mouse on an ongoing basis. |
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