慢性复合应激后FGF-2在成年大鼠海马CA2区的表达变化

目的观察慢性复合应激后大鼠海马神经元FGF-2表达的变化，探讨慢性复合应激对FGF-2表达的影响及其与海马学习记忆功能的联系。方法将成年雄性Wistar大鼠随机分为复合应激组和正常对照组。采用垂直旋转、睡眠剥夺、捆绑和夜间光照四种应激源每天交替无规律刺激复合应激组动物达6周，制作慢性复合应激动物模型。然后利用Morris水迷宫测试大鼠空间学习记忆能力，并运用免疫组织化学方法观察海马区神经元FGF-2表达的变化。结果慢性复合应激后，应激组动物在Morris水迷宫内寻找隐蔽平台所需时间（即潜伏期）较对照组缩短（P〈0．05）；应激组大鼠海马FGF-2免疫反应阳性神经元的表达增多（P〈0．05）。结论慢性复合应激可增强大鼠空间学习记忆能力，而FGF-2在海马神经元的表达增加可能是其影响因素之一。

Expression of FGF-2 in CA2 Subfield of the Adult Rat Hippocampus after Chronic Multiple-stress

Objective To explore the effect of chronic muhiple-stress on learning and memory and the expression of FGF-2 in hippocampal neurons of rats. Methods Adult male Wistar rats were randomly divided into two groups： the multiple stressed group and the control group. Rats in the multiple stressed group were ir- regularly and alternatively exposed to the situation of vertical revolution, sleep deprivation, restraint, and night illumination for 6 weeks to prepare a chronic multiple-stressed model. Then learning and memory performance of rats was measured by Morris water maze and the expression of FGF-2 in hippocampal neurons of rats was de- tected by immunohistochemical method. Results After chronic multiple-stress, compared with the control group, the escape latency to the hidden platform in Morris water maze was shortened in stressed group （ P 〈 0. 05 ）. The expression of FGF-2 in hippocampal neurons of the multiple stressed rats was significantly higher than that of the control group （ P 〈 0.05 ）. Conclusion The increased expression of FGF-2 in hippocampus after chronic multiple-stress may be participated in the enhancing effect of chronic multiple-stress on learning and memory.