瘦素对人牙髓干细胞增殖及分化的影响

目的 探讨瘦素(leptin)对体外培养的牙髓干细胞增殖、分化的影响.方法 体外培养牙髓干细胞,采用MTT法及流式细胞技术检测不同浓度瘦素对牙髓干细胞增殖作用的影响,通过碱性磷酸酶、Yon Kossa染色及矿化相关蛋白(DSP、OCN)的免疫组化染色来检测瘦素对牙髓干细胞分化的影响.结果 瘦素对牙髓干细胞增殖没有显著作用,但是对牙髓干细胞的ALP活性呈浓度依赖性促进.Von Kossa染色可见矿化结节形成,DSP及OCN表达阳性.结论 瘦素可促进牙髓干细胞向成牙本质细胞分化.

Effects of leptin on the proliferation and differentiation of human dental pulp stem cells

Objective To investigate the effects of leptin on proliferation and differentiation of human dental pulp stem cells (hDPSCs).Methods MTT assay and flow cytometric (FCM) were used to evaluate the proliferative effects of leptin in different concentrations. The activity of alkaline phophatase (ALP), Von Kossa and dentin sialoprotein (DSP) and osteocalcin (OCN) were detected to assess the effects of leptin on differentiation of hDPSCs.Results No significant change was observed in the proliferation of hDPSCs after incubation with different concentrations of Lepin compared with the control group. Leptin increased ALP activity of hDPSCs in dose dependently manner. Von Kossa staining showed the formation of mineralized nodules and SABC showed expression of DSP and OCN in hDPSCs after incubation with leptin.Conclusion Leptin did no effects on hDPSCs proliferation, but it can promote hDPSCs to differentiate into odontoblasts.