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Preparation of primary cultures of adult and juvenile catfish (Ictalurus punctatus) hepatocytes |
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| Authors: | Nancy Wohlschlag Laura de los Santos Paula F. Dehn |
| Affiliation: | (1) Division of Life Science, College of Science and Engineering, The University of Texas at San Antonio, 78285 San Antonio, Texas;(2) Biology Department, Canisius College, 2001 Main Street, 14208 Buffalo, NY |
| Abstract: | Summary We describe a modified perfusion and collagenase dissociation in a trypsinizing flask for hepatocyte isolation from adult or juvenile catfish liver. When cells isolated in this manner are allowed to attach to plastic culture dishes in serum-free medium, good viabilities and prolonged function can be obtained. |
| Keywords: | juvenile catfish primary liver cell cultures parenchymal hepatocytes serum-free media adult catfish |
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