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Differentiation of gut endoderm in dependence of the notochord
Authors:Emilie L M J Wiertz-Hoessels  Koki Hara  Johan W M Hekking  Henny W M van Straaten  Frans Thors and Jan Drukker
Institution:(1) Department of Anatomy and Embryology, Medical Faculty, University of Limburg, Maastricht, The Netherlands;(2) Biological Laboratory, Faculty of General Education, Aichigakuin University, Nissin-cho, 470-01 Aichi, Japan;(3) Department of Anatomy and Embryology, University of Limburg, P.O. Box 616, 6200 MD Maastricht, The Netherlands
Abstract:Summary Presumptive intraembryonic endoderm, either isolated or together with adhering mesoderm, from 19-h chick embryos, was grafted to the coelom of 50-h host embryos. The viability of such grafts was low and endodermal differentiation was poor. In a second series the endoderm (with or without adhering mesoderm) was combined with a fragment of notochordal tissue from 48–60-h donor embryos. Then the recovery was much higher, notably after longer periods of in vivo culture. After 10 days of cultivation well-developed entero-endocrine (argyrophilic) cells were found among the regular enterocytes in both series.
Keywords:Endoderm  Entero-endocrine cells  Notochord
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