全身放射损伤对皮肤伤口成纤维细胞增殖的影响

目的 研究合并全身放射损伤时创伤局部成纤维细胞数量减少与细胞增殖的关系。方法 采用组织学和免疫组化方法检测大鼠皮肤伤口局部成纤维细胞数量变化及与细胞增殖密切相关的增殖性细胞核抗原（PCNA）、细胞周期素E（Cyclin E)及细胞周期素依赖性激酶4（CDK4）含量变化。结果 合并全身放射损伤时皮肤伤口局部成纤维细胞数量在伤后5、7和10d显著少于单纯创伤组，并显著延迟高峰期；伤口局部PCNA和CDK4含量在务后3、5和7d显著低于单纯创伤组，伤口局部Cyclin E含量在伤后3、5、7和10d也显著低于单纯创伤组。结论 全身放射损伤可抑制伤后早期细胞周期G1期到S期的转变，抑制细胞增殖，从而使伤口局部成纤维细胞数量减少，这可能是合并全身放射损伤时创伤难愈的重要原因。

Effects of total-body irradiation injury on proliferation of dermal fibroblasts in wounds

Objective To study the relationship between cellular proliferation and the reduction of dermal fibroblasts in wounds combined with total|body irradiation (TBI) injury. Methods We determined the amount of dermal fibroblasts in wounds of rats using histological method,and detected the expression of proliferating cell nuclear antigen (PCNA),cyclin E and cyclin|dependent kinase 4 (CDK4) with immunohistochemistry method. Results The amount of dermal fibroblasts in wounds combined with TBI injury was significantly less than that of simple incision injury on the 5 th ,7 th and 10 th day,and there was a delay of the peak period.The contents of PCNA and CDK4 in wounds combined with TBI injury decreased significantly as compared with those of simple incision injury on the 3 th ,5 th and 7 th day.The contents of cyclin E in wounds combined with TBI injury were also lower than those of simple incision injury on the 3 th ,5 th ,7 th and 10 th day. Conclusion TBI injury suppresses cell transition from G 1 into S phase,inhibits the proliferation of fibroblasts,causing reduction of the amount of dermal fibroblasts in wounds.This may be an important reason why wound heals more slowly when combined with TBI injury.