人参质量的化学模糊模式识别

模拟中医用药习惯制备25个生态环境和生长年数不同的人参样品的水、酒提取液,用紫外—可见分光光度法、高效液相色谱法和电感偶合等离子体发射光谱法获取反映提取液内化学成分整体的数据。再用抗疲劳作用、抗利尿作用和巨噬细胞吞噬功能测定提取液的生物活性。然后以药理实验的结果为依据对上述化学数据进行特征抽取,求解模糊关系方程,以探明人参疗效权重不同的有效成分和它们的配伍关系。最后通过成分的相关分析,制定出一个评价人参质量的新方案。所提出的方法学对中药质量的评价和中药有效成分的确定具有普遍意义。

QUALITY ASSESSMENT OF GINSENG BY CHEMICAL FUZZY PATTERN RECOGNITION

Water and liquor extracts of ginseng simulating its habitual prepara-tions used in the practice of traditional Chinese medical science were prepared from 25samples, and data reflecting its chemical constituents as a whole were obtained byultraviolet and visible spectrophotometry (UV-Vis), high performance liquidchromatography(HPLC), and emission spectroscopy with inductively coupledplasma source (ICP). Antifatigue, antidiuretic, and macrophage phagocytosis experimen-ts were carried out to determine the pharmacologic actions of the extracts. Results obtainedwith the extracts on mice were compared mutually and with those obtained on placebo-treated ones in the respective pharmacologic experiments. Based on the results of the threepharmacologic experiments ,features were selected from among the chemical data and fuzzyequations formulated hereby were solved to ascertain the effective constituents withdifferent weights and their compatibility. Finally, correlation analysis was carried out,anda new scheme for the quality assessment of ginseng has been worked out. Themethodology proposed is of general significance to the formulation of quality assessmentschemes for Chinese traditional medicines and the ascertainment of their bioactive consti-tuents.