类风湿关节炎类风湿因子阴性和阳性寒热证候患者外周血CD4+T细胞基因差异表达研究

目的探讨类风湿因子阴性和阳性类风湿关节炎寒热证候患者外周血CD4^＋ T细胞的基因表达差异。方法采集类风湿关节炎寒热证候患者及健康人空腹静脉血，纯化得到CD^4＋T细胞，利用基因芯片检测和分析技术，探索类风湿因子阴性和阳性类风湿关节炎寒热证候患者CD^4＋T细胞基因表达差异点。结果类风湿因子阴性和阳性类风湿关节炎患者之间有55条基因表达存在显著性差异，主要涉及免疫应答和信号传导；类风湿因子阳性类风湿关节炎寒热证候患者之间有71条基因异常表达，主要涉及功能代谢和免疫应答；类风湿因子阴性类风湿关节炎寒热证候患者之间有70条基因异常表达，没有与上述类风湿因子阴性和阳性患者之间55条基因重复，与类风湿因子阳性寒热证候之间的71条基因只有2条基因重复，主要涉及功能代谢。结论类风湿关节炎患者类风湿因子阴性和阳性之间的基因表达谱差异与寒热证候之间的基因表达差异有所不同，提示中医证候分类学具有基因表达谱依据。

Study on the Differential Gene Expression of Peripheral CD4+ among Rheumatoid Arthritis Patients of Cold or Heat Syndrome Type with or without Rheumatoid Factor

ObjectiveTo explore the differential gene expression of peripheral CD~ _4 among rheumatoid arthritis (RA) patients of cold or heat syndrome type with or without rheumatoid factor (RF). MethodsDifferential gene expression of peripheral CD~ _4 lymphocytes purified from fasting venous blood of RA patients and healthy subjects was studied using gene chip technique. ResultsThere were 55 differential genes between RA patients with and without RF, mainly involving those related with immune response and signal transduction. In patients with RF, 71 differential genes, mainly related with functional metabolism and immune response, and in those without RF, 70 related with functional metabolism were found between patients of cold and heat syndrome type respectively, all of them were not repetitive with the above-mentioned 55, and only 2 were found repetitive between the 70 and the 71 differential genes, mainly involving functional metabolism. ConclusionThe differential genes between RA patients with or without RF are different with those between patients of heat and cold syndrome type, suggesting the TCM syndrome classification has its own basis of gene expression profile.