慢性神经病理性疼痛对大鼠脊髓背角miRNA表达的影响

目的 筛选慢性神经病理性疼痛大鼠脊髓背角差异表达的miRNA,并预测其调控的靶基因.方法 建立大鼠坐骨神经慢性压迫损伤CCI模型,在术后疼痛高峰期取腰膨大脊髓背角,用miRNA芯片筛选CCI大鼠差异表达的miRNAs,再用荧光实时定量RT-PCR验证差异表达的miRNAs,并利用MIRANDA、TARGETSCAN、PICTAR 3个数据库找出这些miRNA可能调控的靶基因.结果 CCI大鼠表达上调的有miR-99b,表达下调的有miR-674-3p、miR-879与miR-325-5p.RT-qPCR验证结果与芯片基本相符.预测这些miRNA可能的靶基因约26个,这些基因功能广泛.结论 慢性神经病理性疼痛可导致miRNA的表达发生变化,这些miRNA及其调控的靶基因为进一步研究奠定了基础.

Effect of chronic neuropathic pain on the expression of microRNA in the spinal dorsal horn of rats

Objective To investigate the differentially expressed miRNAs in the spinal dorsal horns of rats with chronic neuropathic pain, and predict the target genes of the miRNAs. Methods CCI(Chronic Constrictive Injury) rat model was established，and spinal dorsal horns were harvested when a peak pain was achieved on the 7th day post-op. Microarray analysis was applied to investigate the differentially expressed miRNAs in CCI rats, and the screened miRNAs were confirmed by real time RT-PCR. Furthermore, the possible target genes were predicted by three databases. Results miRNA microarray analysis showed the expression of miR-99b as significantly up-regulated, while the expressions of miR-674-3p，miR-879 and miR-325-5p as significantly down-regulated in the spinal dorsal horns of CCI rats compared with those in the sham and na?ve rats. The different expression profiles of the 4 miRNAs were confirmed by quantitative RT-PCR. About 26 target genes were predicted through the 3 databases based on the previous screened miRNAs. Conclusions Chronic neuropathic pain affecedt the expression of miRNAs. These miRNAs and target genes provided new clues for further research.