甲醛暴露工人XRCC1基因多态性与DNA损伤的关系研究

目的探讨甲醛暴露工人DNA修复基因XRCC1多态性与外周血淋巴细胞DNA损伤的关系。方法选择某密度板厂的151名甲醛暴露工人(暴露组)和某推土机厂的112名非甲醛暴露工人(对照组)为研究对象。用气相色谱法检测作业环境的甲醛浓度,应用彗星实验测定研究对象外周血淋巴细胞DNA损伤,以Olive尾距和彗星尾长反映DNA损伤水平,用PCR-RFLP方法分析XRCC1基因的多态性;用多元协方差分析调整工人的年龄、工龄、职业甲醛暴露及吸烟与饮烟情况,比较XRCC1基因不同基因型个体的Olive尾距和彗星尾长。结果使用多元协方差分析校正甲醛暴露工人的年龄、工龄、甲醛暴露水平和吸烟与饮酒情况后,携带Arg280His位点变异基因型个体的Olive尾距和彗星尾长(几何均值分别为4.30和13.42)均显著高于野生型基因型的个体(几何均值分别为3.38和11.71),差异均有显著性(Olive尾距:P<0.05,彗星尾长:P<0.01);未发现XRCC1基因其他3个位点的多态性与甲醛暴露工人Olive尾距和彗星尾长有显著关联。结论XRCC1基因Arg280His位点的多态性影响甲醛暴露工人的DNA损伤水平。

Association between XRCC1 gene polymorphisms and DNA damage of workers exposed to formaldehyde

Objective To investigate the association between polymorphisms of DNA repair gene XRCC1 and DNA damage in peripheral blood lymphocytes among workers exposed to formaldehyde.Methods One hundred and fifty-one workers exposed to formaldehyde from plywood factories and one hundred and twelve workers without occupational exposure to formaldehyde were recruited into this study.DNA damage levels were measured by comet assay.The polymorphisms of XRCC1 gene were analyzed by polymerase chain reaction(PCR) with restriction fragment length polymorphism(RFLP) method.The multiple covariance analysis was used to compare olive trail moment and comet trail length adjusted confounding factors.Results In formaldehyde exposed workers,after ages,smoking and drinking status and occupational exposure level were adjusted,means of Olive trail moment and comet trail length in the subjects with variant genotype at Arg~(280)His site(geometric means 4.30 and 13.42 respectively) were higher than subjects with wild type homozygote(geometric means 3.38 and 11.71 respectively),the differences were significant(Olive trail moment:P<0.05,comet trail length:P<0.01).No associations between the polymorphisms at other three sites in XRCC1 gene and means of olive trail moment and comet trail length in exposure workers were found.Conclusion The polymorphisms of XRCC1 gene may modulate the effects of DNA damages induced by formaldehyde in workers.