重组降血压肽缓释微球对自发性高血压大鼠的降压作用

目的考察重组降血压肽聚乳酸(rAHP-PLA)缓释微球对自发性高血压大鼠(SHR)的降压作用。方法采用口服和皮下注射两种给药方式,分别给予按体质量和血压均衡分组的自发性高血压大鼠rAHP-PLA缓释微球,空白组给予等量生理盐水。结果口服给予降血压肽缓释微球后10 h,自发性高血压大鼠的血压降至最低,且药效可以持续36 h;皮下注射rAHP-PLA缓释微球后30 h,血压降至最低,且可以持续约9 d。rAHP-PLA缓释微球对正常血压无影响。结论 rAHP-PLA缓释微球具有显著的降压效果,并有明显的缓释作用。

Antihypertensive effects of biodegradable microspheres loaded with recombinant antihypertensive peptide on spontaneously hypertensive rats

Purpose To study the effect of biodegradable microspheres loaded with recombinant antihypertensive peptide(rAHP) on blood pressure of spontaneously hypertensive rats.Methods SHR were randomized into ten groups(n=8,each group) and received one of the following approches by gavage and subcutaneous injection respectively:saline,rAHP(800 μg/kg),low-dose(200 μg/kg),medium-dose(400 μg/kg) and high-dose(800 μg/kg) of the rAHP-PLA microspheres.Thirty-two WKY rats were randomized into four groups(n=8,each group),and rAHP-PLA microspheres(800 μg/kg) and saline were administrated by gavage and subcutaneous injection respectively.The systolic blood pressure was measured before and after the administration.Results The results showed that compared with the control group,medium-dose and high-dose of rAHP-PLA microspheres reduce blood pressure after being orally administrated and the effect can last for above 36 hours.For the subcutaneous injection groups,medium-dose and high-dose of rAHP-PLA microspheres reduce blood pressure and the effect can last for above 9 days.rAHP-PLA microspheres had no effect on reducing blood pressure in WKY rats.Conclusion The anti-hypertensive effects of the rAHP-PLGA microspheres persist for much longer time,with no effect on normotensive WKY rats.Conclusion The anti-hypertensive effects of the rAHP-PLA microspheres persist for much longer time,with no effect on normotensive WKY rats.