白细胞介素5调节变应性鼻炎骨髓反应的实验研究

目的　探讨白细胞介素 5 (interleukin 5 ,IL 5 )对变应性鼻炎动物模型骨髓反应的调节作用和机制。方法　采用 6～ 8周雄性SD大鼠 ,随机分成实验组 10只和对照组 10只 ,以卵清蛋白致敏激发制成变应性鼻炎模型 ,瑞氏染色计数骨髓涂片和外周血涂片中白细胞比例 ,免疫组化染色检测骨髓中IL 5的表达。结果　大鼠变应性鼻炎模型骨髓涂片中嗜酸粒细胞、嗜碱粒细胞比例显著高于对照组 (P <0 0 1) ,变应性鼻炎模型外周血涂片中嗜酸粒细胞比例显著高于对照组 (P <0 0 1) ,变应性鼻炎模型骨髓涂片中IL 5阳性细胞比例显著高于对照组 (P <0 0 5 ) ,变应性鼻炎模型骨髓涂片中IL 5阳性细胞比例和嗜酸粒细胞比例呈显著正相关 (R =0 85 ,P <0 0 1)。结论　骨髓IL 5的表达能促进嗜酸粒细胞增生和迁徙 ,抑制骨髓中IL 5介导的嗜酸粒细胞的增殖和分化 ,可能开辟治疗炎症新方法。

Interleukin-5 regulate the bone marrow response in the model of allergic rhinitis

Objective To elucidate the mechanism of bone marrow response regulated by interleukin-5 (IL-5) in the model of allergic rhinitis. Methods Twenty SD rats were randomly divided into allergic rhinitis(AR) group and control group.The leucocytes in the smears of bone marrow and peripheral blood were counted, and the expression of IL-5 was detected by immunohistochemistry. Results The ratio of eosinophils to white cells in bone marrow smears of AR group was significantly higher than that of control group( P <0.01).The ratio of basophils to white cells in bone marrow smears of AR group was significantly higher than that of control group( P <0.01).The ratio of eosinophils to white cells in peripheral blood smears of AR group was significantly higher than that of control group( P <0.01).The ratio of IL-5 positive cells to white cells in bone marrow smears of AR group was significantly higher than that of control group( P <0.05).The ratio of IL-5 positive cells to white cells was significantly positively correlated with the ratio of eosinophils to white cells in bone marrow smears of AR group( R =0.85, P <0.01). Conclusion IL-5 regulates the proliferation and differentiation of eosinophils in bone marrow.