罗格列酮对高脂血症家兔低密度脂蛋白氧化的影响

目的研究罗格列酮对高脂血症家兔低密度脂蛋白氧化的影响。方法采用高脂饮食建立家兔高脂血症模型,观察罗格列酮对血清脂质水平、血清总抗氧化能力(TAC)及血清氧化低密度脂蛋白(Ox-LDL)水平的影响;超速离心法分离低密度脂蛋白(LDL),采用铜离子进行氧化,观察罗格列酮对LDL氧化易感性的影响。结果罗格列酮可显著提高高脂血症家兔血清TAC,增强LDL抗氧化能力,降低血清Ox-LDL水平,但对血清总胆固醇(TC)等脂质水平无明显影响。结论罗格列酮可有效抑制高脂血症家兔LDL氧化,其机制与提高血清总抗氧化能力、降低LDL氧化易感性有关。

Effect of Rosiglitazone on low-density lipoprotein oxidation in hyperlipidemic rabbits

Objective To investigate the effect of rosiglitazone on low-density lipoprotein(LDL) oxidation in hyperlipidemic rabbits.Methods Twenty-four male New Zealand white rabbits were allocated randomly to three groups fed on standard diet,high lipid diet or high lipid diet supplemented with rosiglitazone(50 mg/kg) for 4 weeks,respectively.Serum levels of lipids,oxidized LDL(Ox-LDL) and total antioxidant capacity(TAC) were determined at the end of experiment.Following isolation by ultracentrifugation,LDL of different groups was subjected to cupric ion-induced oxidation.Results Rosiglitazone significantly increased serum TAC in hyperlipidemic rabbits,while LDL oxidizability and serum concentration of Ox-LDL were decreased markedly.Rosiglitazone had no significant influence on serum levels of total cholesterol,triglyceride,LDL and high-density lipoprotein.Conclusion Rosiglitazone could effectively suppress LDL oxidation in hyperlipidemic rabbits,which might be attributed,at least partly,to the increased resistance of LDL to oxidation.