凝血质控品复溶后稳定性研究

目的通过对凝血质控品复溶后冷冻保存不同时间PT、APTT、TT的检测,探讨凝血质控品复溶后冷冻保存对其稳定性的影响。方法每周一取1瓶干粉状凝血质控品复溶后分装于5支具盖离心管中,留1支当天检测,其余4支-20℃冷冻保存,于周二至周五毎天均取1支,快速复溶后随标本一起进行质控测试,测定结果填写到质控图中。结果凝血质控品复溶后PT、APTT、TT的日间CV值均小于5%,分装后第2～5 d的测定结果与开瓶当日的测定结果间差异无统计学意义(P0.05)。结论凝血质控品复溶后-20℃冷冻保存5 d内测定结果稳定。

Stability of coagulation quality control materials after reconstitution

Objective To explore the effects of stability of coagulation control materials after reconstitution through PT,APTT,TT testing at different times after the cryopreservation.Methods A bottle of dry powder coagulation control materials was taken at Monday,reconstituted and packed in 5 cover tubes,leaving one for testing at once,and the remaining were cryopreserved at-20℃ for Tuesday to Friday testing,and the results were filled out in the quality control chart.Results CV values among day of PT,APTT,TT in coagulation control materials after reconstituted were less than 5%,and the results showed no significant difference between 2~5 days after packing and the date of the determination(P>0.05).Conclusion The measurement results of coagulation control materials are stable in 5 days of-20℃ cryopreservation after reconstituted.