| 多巴胺对鱼藤酮细胞毒性介导作用研究 |
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| 引用本文： | 胡丹,白寅又,张兆辉,孙圣刚. 多巴胺对鱼藤酮细胞毒性介导作用研究[J]. 脑与神经疾病杂志, 2010, 18(4) |
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| 作者姓名： | 胡丹 白寅又 张兆辉 孙圣刚 |
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| 作者单位： | 1. 武汉大学人民医院神经内科,湖北,430060
2. 华中科技大学同济医学院附属协和医院神经内科 |
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| 摘 要： | 目的探讨多巴胺在鱼藤酮细胞毒性损伤中的作用。方法采用鱼藤酮(1μmol/L)处理PC12细胞,噻唑蓝比色法检测细胞活性,流式细胞术检测DHR123荧光强度;并采用多巴胺耗竭剂-利血平预处理3小时,观察上述指标。结果 1μmol/L鱼藤酮处理PC12细胞24小时,导致细胞活力较正常组显著下降(P<0.05),吸光度为0.730±0.01(正常组为1.112±0.025);利血平(1μmol/L和5μmol/L)预处理后再给予鱼藤酮,孵育24小时后,吸光度分别为0.945±0.02和1.06±0.03,细胞活力较鱼藤酮组显著升高(P<0.05)。鱼藤酮处理24小时,过氧化物水平升高至正常组的281%,而利血平(1μmol/L和5μmol/L)预处理后,分别降至正常组的248%和232%,与鱼藤酮组比较有显著差异(P<0.05)。结论多巴胺介导了鱼藤酮对多巴胺能细胞的毒性作用。
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| 关 键 词： | 多巴胺 鱼藤酮 帕金森病 |
A study of the role of Dopamine in the neurotoxicity of rotenone |
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| HU Dan,BAI Yin-you,ZHANG Zhao-hui,SUN Sheng-gang. A study of the role of Dopamine in the neurotoxicity of rotenone[J]. Journal of Brain and Nervous Diseases, 2010, 18(4) |
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| Authors: | HU Dan BAI Yin-you ZHANG Zhao-hui SUN Sheng-gang |
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| Affiliation: | HU Dan; BAI Yin-you; ZHANG Zhao-hui; SUN Sheng-gang.Department of Neurology; the People s Hospital of Wuhan University; Wuhan 430060; China; |
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| Abstract: | Objective To explore the role of dopamine in the neurotoxicity of rotenone.Methods PC12 cells were treated with 1μmol/L rotenone for 24 hours.MTT assay was used to determine the cell viability.Intracellular reactive oxygen species were measured by the fluorescence probe of dihydrorhodamine 123(DHR123)with flow cytometry.Pretreatment of reserpine(1μmol/L or 5μmol/L)for 3 hours was also performed,and the above indexes were analysed.Results 24h-treatment with rotenone at 1μmol/L dramatically decreased the cell... |
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| Keywords: | Dopamine Rotenone Parkinson disease |
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