Chrysoeriol isolated from Eurya cilliata leaves protects MC3T3-E1 cells against hydrogen peroxide-induced inhibition of osteoblastic differentiation

Chrysoeriol is a flavonoid compound found in several tropical medicinal plants. To elucidate the protective effects of chrysoeriol isolated from Eurya cilliata on the response of osteoblasts to oxidative stress, osteoblastic MC3T3‐E1 cells were incubated with chrysoeriol and/or H₂O₂, and markers of osteoblast function and oxidative damage were examined. Chrysoeriol treatment significantly (P < 0.05) reversed the cytotoxic effect of H₂O₂ and increased collagen content, alkaline phosphatase activity and calcium deposition of osteoblasts in the presence of H₂O₂. These effects were blocked by ICI182780, suggesting that chrysoeriol's effect might be partly involved in estrogen action. Moreover, H₂O₂‐induced reduction of osteocalcin was recovered in the presence of chrysoeriol. Chrysoeriol significantly (P < 0.05) decreased the production of receptor activator of nuclear factor‐κB ligand, interleukin‐6, protein carbonyl and malondialdehyde of MC3T3‐E1 cells in the presence of H₂O₂. These results demonstrate that chrysoeriol isolated from E. cilliata can protect osteoblasts from oxidative stress‐induced toxicity. Copyright © 2010 John Wiley & Sons, Ltd.