| Bax基因对人胃癌耐药细胞多药耐药性的逆转作用 |
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| 引用本文： | 赵燕秋,肖冰,樊代明. Bax基因对人胃癌耐药细胞多药耐药性的逆转作用[J]. 中华消化杂志, 2000, 20(1): 8-10 |
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| 作者姓名： | 赵燕秋 肖冰 樊代明 |
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| 作者单位： | 1. 710032,西安,第四军医大学西京医院消化科
2. 第一军医大学南方医院消化科 |
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| 基金项目： | 国家自然科学基金杰出青年基金!资助项目 (3952 50 2 0 ) |
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| 摘 要： | 目的 研究凋亡相关基因Ｂax对胃癌多药耐药细胞的多药耐药性的逆转作用。方法 构建含有人Ｂax cＤＮＡ全长的真核表达载体pＢＫ－Ｂax，经脂质体导入缺乏Ｂax蛋白表达的人 胃癌多药耐药细胞系ＳＧＣ７９０１/ＶＣＲ中。Ｗestern印迹观察Ｂax基因在转导细胞中的表达，ＭＴＴ法检测Ｂax转导细胞与空载体转导细胞对化疗药物的敏感性。结果 成功构建了Ｂax的真核表达载体，Ｂav转导细胞能稳定表达Ｂax蛋白，与空载体转导细胞相比。Ｂ
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| 关 键 词： | Bax基因 转导 胃癌 多药耐药 药物疗法 基因治疗 |
Reversal of multidrug resistance of gastric cancer cell line SGC7901/VCR by Bax gene transduction |
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| ZHAO Yanqiu,XIAO Bing,FAN Daiming. Reversal of multidrug resistance of gastric cancer cell line SGC7901/VCR by Bax gene transduction[J]. Chinese Journal of Digestion, 2000, 20(1): 8-10 |
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| Authors: | ZHAO Yanqiu XIAO Bing FAN Daiming |
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| Abstract: | Objective To investigate the effect of Bax gene transduction on the multidrug resistance of gastric cancer cell line SGC7901/VCR. Methods Construct a eukaryotic vector carrying the full length of Bax cDNA, then transfect it into SGC7901/VCR cells lacking Bax protein expression by lipofectamine. After G418 selection, Western blot was used to detect the Bax protein expression in Bax transducted cells; the drug sensitivity between Bax transfectants and vector transducted cells were compared with MTT assay only. Results The authors successfully constructed Bax eukaryotic expression vector. Bax protein was stably expressed in Bax transfectants. MTT results showed Bax transfectants were more sensitive to VCR, ADR and MMC than vector transducted cells singly. Conclusion Bax gene transduction reversed the multidrug resistance of gastric cancer cell line SGC7901/VCR to some extent. |
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| Keywords: | Bax gene Transduction Gastric cancer Multidrug resistance |
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