硫喷妥钠或异丙酚麻醉不同时期大鼠脑ATP酶和NO合酶活性以及NO生成量的动态变化

目的:观察硫喷妥钠或异丙酚麻醉不同时期,大鼠皮层、脑干Ca2 ATPase、Na ,K ATPase活性、NOS活性和NO生成量的动态变化。方法:大鼠腹腔注射硫喷妥钠30mg·kg-1或异丙酚10 0mg·kg-1,以翻正反射消失为标准,分别于诱导期、麻醉期,恢复期和苏醒期断头取脑(n =8) ,分离出皮层和脑干,测定Ca2 ATPase、Na ,K ATPase、NOS的活性以及NO的生成量,腹腔注射生理盐水10ml·kg-1作为对照组。结果:大鼠皮层、脑干Ca2 ATPase、Na ,K ATPase的活性在硫喷妥钠诱导期、麻醉期和恢复期显著性降低(P <0 .0 5或P <0 .0 1) ,在异丙酚麻醉期亦显著性降低(P <0 .0 5 )。腹腔注射硫喷妥钠或异丙酚后,NOS活性和NO生成量从诱导期到恢复期均显著性降低(P <0 .0 1) ,在苏醒期可恢复至对照水平。结论:Ca2 ATPase、Na ,K AT Pase、NOS活性及NO的产生介导了硫喷妥钠或异丙酚的麻醉作用。

Dynamic changes of ATPases and NOS activities and NO production at different anesthesia phases of thiopental and propofol anesthesia

AIM: To investigate the dynamic changes of ATPases and NOS activities and NO production at different anesthesia phases using thiopental and propofol andifferent anesthetic phases (induction, anesthesia, restoration, and awake), the activities of NOS and ATPase and NO production in cortex and brain stem were meagroup. RESULTS: Ca2+ -ATPase and Na+ ,K+ -ATPase activities in the cortex and brain stem were significantly decreased after administration ofthiopental and propofol,especially at induction, anesthesia, or even restoration phase of thiopental group (P＜0.05, P＜0.01) and at anesthesia phase of propofol group (P＜0.05). NOS activities and NO production decreased from induction to restoration phase with thiopental and propofol anesthesia (P＜0.01). The parameters were returned near to the normal at awaken phase. CONCLUSION: Activities of ATPases and NOS and the production of NO may mediate the anesthesia effects of thiopental and propofol in the rat cortex and brain stem.