麻醉大鼠肠淋巴循环的研究

探讨肠淋巴循环的研究方法、参数及正常值，为淋巴学研究提供参考。方法：通过微循环显微电视技术，对７５只大鼠进行肠系膜淋巴微循环研究，同时采用肠淋巴管插管方法对６０只大鼠实施肠淋巴管插管，测定大鼠肠淋巴流量、淋巴蛋白输出量和肠淋巴管压力。结果：麻醉大鼠肠系膜淋巴管静态口径为１０３．８９± ７．１８μｍ，自主收缩频率为６０．１± ０．２２次／ｍｉｎ，淋巴管收缩分数（ＩｎｄｅＩ）为０．５６± ０．０３，总收缩活性指数（Ｉｎ－ｄｅｘⅡ）为１．７７±０．０９，肠淋巴流量为１０．７４±０．４９μｌ／ｍｉｎ，肠淋巴管压力为１．８５± ０．１７ ｋＰａ。结论：肠系膜淋巴管口径、收缩性参数及淋巴流量的测定，可用于淋巴循环的研究。

Study on Intestinal Lymph Circulation in Anesthetized Rats

Objective: To discuss the research method, parameters and their normal values about the intestinal lymph circulation and so as to provide some refer- ences for the research of lymphology. Metghod: A study of mesenteric lymph microcirculation was done in 75 rats by a microscope video-recorder tech nique, and the same time, a cannula of intestinal lymph duct was also done in 60 rats for the determination of intestinal lymph flow, lymph protein output and lymph pram. Results: In anesthetized rats, the diameters of the first grade arteriole and venule was 22. 77 ± 0. 68 μm and 31. 79 t 0. 92 μm and these of the second grade was 15. 75 ± 0. 49 μm and 22. 84 ± 0. 88 μm, sepa- rately. The still diaxneter of mesenteric lymph vessel was 103. 89 ± 7. 18 μm, the contracted frequency was 6. 01 ± 0. 22 time. min- 1; Index I was 0. 56 ±0. 03, Index Ⅱ was 1. 77 ± 0. 09, L. D-Index was 3. 70 ± 0. 18. The in- testinal lymph flow, lymph protein output and lymph pressure was respec- tively 10. 74 ± 0. 49 μl/win, 386. 09 ± 18. 71 μg/min and 1. 85 ± 0. 17 kPa. Conclusion: The diameter of mesenteric lymph vessel, contracted parameters and intestinal lymph flow could use in the reseach of intestinal lymph circula- tion.