Inhibitory effect of high dose ascorbic acid on inflammatory edema

The ability of ascorbic acid (AA) as an antioxidant to suppress the inflammatory reaction was investigated. Carrageenan-induced foot pad edema was produced in the right hind foot of anesthetized mice (n = 22). Subsequently, Group A (n = 11) received 25 mg AA in saline (IP) and Group B, an equal volume of saline. After 2 1/2 hrs the animals were sacrificed and increase in weight of the amputated right paw over the amputated left paw was expressed as percentage edema (PE). The PE in Group A was 43.8 +/- 5.9, and in Group B was 59.3 +/- 3.9 (p less than 0.05, unpaired t-test). The same experiment was repeated with the AA administered 10 minutes prior to injury. The change in edema was not statistically significant. It is concluded that high dose AA suppresses edema if given after but not before injury.