大鼠创伤性脑损伤后胰岛素抵抗的研究

目的:探讨大鼠创伤性脑损伤后是否存在胰岛素抵抗现象.方法:采用大鼠自由落体脑损伤模型(Feeney'smodel),分别在伤前1/2h及伤后6、12、24、48、72、120h测定轻、中、重型脑损伤动物的血糖和血清胰岛素值.运用正常血糖-高血胰岛素钳夹技术,检测大鼠重型创伤性脑损伤后24h后BG60-120、GIR60-120、ISI等3个反映胰岛素敏感性的指标.结果:中、重型创伤性脑损伤后大鼠血糖含量升高的同时血清胰岛素水平升高,大鼠重型创伤性脑损伤后24hBG60-120显著地升高,GIR60-120、ISI显著地降低.结论:在重型创伤性脑损伤急性期,大鼠血糖和血清胰岛素水平均显著地升高,高水平的胰岛素未能起到相应的降低血糖的作用,与机体产生胰岛素抵抗有关.

The insulin resistance after traumatic brain injury in rats

Objective:To study the insulin resistance after traumatic brain injury in rats.Methods:Based upon the Feeney's model,the blood glucose and insulin concentration of each animal group were measured 1/2 hour before injury and 6,12,24,48,72,120 hours after injury;the three indices BG_(60-120),GIR_(60-120),ISI reflecting the insulin sensitivity were measured 24 hours after severe traumatic brain injury by euglycemic-hyperinsulinemic clamp technique.Results:Both the blood glucose and insulin concentration increased markedly in medium and severely injured group after injury.BG_(60-120) increased markedly and GIR_(60-120),ISI decreased significantly 24 hours after severe trauma in injured animal compared with sham group.Conclusion:Both the blood glucose and insulin concentration increase markedly in severe injured group after injury.The fact that higher-level insulin cannot reduce the increased blood glucose is due to acute insulin resistance developing after traumatic brain injury.