塞替派、环磷酰胺诱发的人支气管上皮恶性转化细胞的致瘤性

目的　观察塞替派 (TE)及环磷酰胺 (CP)能否诱发永生化人支气管上皮恶性转化细胞成瘤。方法以永生化人支气管上皮细胞 (BEAS 2B)为对照 ,以TE诱发转化细胞 (BEAS TE)的琼脂糖克隆扩增细胞(BEAS STE)及CP诱发转化细胞 (BEAS CP)的琼脂糖克隆扩增细胞 (BEAS SCP)为靶细胞接种于 3～ 4周龄裸鼠 ,每组接种 6只 ,♂♀各半。结果　接种后2 6周 ,对照组无一长出肿瘤 ,BEAS STE组有 5只长出肿瘤 ,BEAS SCP组有 1只长出肿瘤 ,其中BEAS STE组有 3只裸鼠的肿瘤直径在 1cm以上。经病理组织形态和免疫组织化学检查证实肿瘤为低分化癌肉瘤组织。结论　TE、CP诱发恶性转化的人支气管上皮转化细胞均具有裸鼠致瘤性。

Tumorigenicity of immortalized human bronchial epithelial celltransformed by thiotepa or cyclophosphamide

AIM To determine the tumorigenic potential of immortalized human bronchial epithelial cell malignantly transformed by thiotepa(BEAS-TE) and cyclophosphamide(BEAS-CP) in nude mice. METHODS The BEAS- TE/BEAS-CP cells selected from agarose (BEAS-STE/BEAS-SCP) were expanded and xenotransplanted into 3-4 week- old nude mice. The immortalized human bronchial epithelial cell(BEAS- 2B) which can not form foci in agarose was used as control. RESULTS Twenty- six weeks after transplantation, 5 of 6 nude mice injected with BEAS- STE cells and 1 out of 6 nude mice injected with BEAS- SCP cells developed tumors, while no tumor was formed in the six control animals. Histopathological examination and immunohisto-chemical staining of cytokeratin and vimentin of the tumor tissues indicated that the tumors were carcinosarcoma in nature. CONCLUSION The immortalized human bronchial epithelial cells malignantly transformed by thiotepa/cyclophosphamide have the tumorigenic potential in nude mice.