| 抗人tPA单克隆抗体的制备 |
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| 引用本文： | 张代新,徐德胜,张晓燕,刘娜新,赵海霞. 抗人tPA单克隆抗体的制备[J]. 北京大学学报(医学版), 1996, 0(1) |
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| 作者姓名： | 张代新 徐德胜 张晓燕 刘娜新 赵海霞 |
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| 作者单位： | 北京医科大学细胞生物学教研室 |
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| 摘 要： | 目的：制备抗ｔＰＡ单克隆抗体，用于建立ＥＬＩＳＡ方法和研究ｔＰＡ与肿瘤转移的关系。方法：用基因重组的组织型纤溶酶原激活剂（ｒｔＰＡ）免疫Ｂａｌｂ／ｃ小鼠，按常规法作细胞融合，用ＥＬＩＳＡ法筛选出阳性反应的杂交瘤细胞克隆。结果：获得一株杂交瘤细胞株（２Ｂ７）；其免疫球蛋白亚类为ＩｇＧ１；经ＥＬＩＳＡ法和免疫印迹技术证明，此单抗能特异性识别ｔＰＡ。结论：用２Ｂ７ＭｃＡｂ特异性强，用其建立的夹心ＥＬＩＳＡ检测ｔＰＡ的方法线性较好，灵敏度可达１ｎｇ／ｍｌ。２Ｂ７ＭｃＡｂ可望用于ｔＰＡ与肿瘤转移关系及导向溶栓的研究。
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| 关 键 词： | 抗体，单克隆；肿瘤转移；纤维蛋白溶酶原激活物，组织型 |
PREPARATION AND CHARACTERIZATION OF tPA MONOCLONAL ANTIBODY |
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| ZHANG Daixin,XU Desheng,ZHANG Xiaoyan,LIU Naxin,ZHAO Haixia. PREPARATION AND CHARACTERIZATION OF tPA MONOCLONAL ANTIBODY[J]. Journal of Peking University. Health sciences, 1996, 0(1) |
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| Authors: | ZHANG Daixin XU Desheng ZHANG Xiaoyan LIU Naxin ZHAO Haixia |
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| Abstract: | Objective:In order to prepare anti-tPA monoclonal antibody,which is used to study the role of tPA in tumor metastasis.Methods:Babl/c was immunized peritoneally with rtPA and complete con-jugate.Then,immunized spleen cells and SP2/0 were fused in the help of PEG,Positive clones were selected with ELISA. Results:One hybridoma(2B7)secreting specific anti-tPA McAb was prepared.The McAb is subclassed into IgG1.Conclusions:2B7 McAb react specifically with both recombinant and natural tPA,but not with irrelevant proteins,ELISA kit established with 2B7 McAb showed high sen-sitivity and specificity. |
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| Keywords: | Headings Antibodies monoclonal Neoplasm metastasis Plasminogen activator tissue-type |
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