电磁辐射对PC12细胞GluR2蛋白质表达及磷酸化的影响

目的 探讨电磁辐射对PC12细胞GluR2蛋白质表达及蛋白质磷酸化水平的影响。方法 对离体培养的PC12细胞给予平均功率密度为90mW/cm²的电磁波持续辐照20min,采用western blot方法检测辐照后0h、3h、12h、24h四个时相点GluR2蛋白质表达及蛋白质磷酸化水平的变化。结果 电磁波辐照后0h和3hGluR2的蛋白质表达水平降低(P<0.05),其余各时相点无显著变化;辐照后0hGluR2的蛋白质磷酸化水平显著降低(P<0.01),3h后逐渐恢复至正常水平。结论 平均功率密度为90mW/cm²的电磁波持续辐照20min能够降低PC12细胞GluR2的蛋白质表达和蛋白质磷酸化水平。

Effects of Electromagnetic Irradiation on the Level of GluR2 Protein Expression and Phosphorylation in PC12 Cells

Objective To observe the effects of electromagnetic irradiation on the level of GluR2 protein and phosphorrylation in PC12 cells.Methods The cultured PC12 cells were exposed to 90 mW/cm2(average power density)electromagnetic wave for 20 min,The level of GluR2 protein and phosphorylation of PC12 cells were examined by western blot at 0 h,3 h,12 h,24 h after irradiation.Results The level of GluR2 protein of PC12 cells decreased at 0 h and 3 h after irradiation;The level of GluR2 phosphorylation of PC12 cells decreased significantly at 0 h after irradiation.Conclusion 90 mW/cm2(average power density)electromagnetic wave could lower the level of GluR2 protein and phosphorylation in PC12 cells.