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金银花醇提液对根管粪肠球菌生物膜作用体外研究
引用本文:许颖,李德超,孙剑.金银花醇提液对根管粪肠球菌生物膜作用体外研究[J].黑龙江医药科学,2010,33(6):49-50.
作者姓名:许颖  李德超  孙剑
作者单位:佳木斯大学附属口腔医院牙体牙髓病科,黑龙江佳木斯154002
基金项目:黑龙江省教育厅科学技术研究项目
摘    要:目的:验证金银花醇提液对粪肠球菌生物膜的抑制作用。方法:建立粪肠球菌生物膜体外模型,M TT法计算金银花醇提液对粪肠球菌生物膜的抑菌率。并利用激光共聚焦显微镜(CLSM)观察药物对生物膜中粪肠球菌的影响。结果:金银花醇提液对粪肠球菌生物膜的抑制作用随药物浓度增加而增加。CLSM观察见金银花醇提液可使粪肠球菌生物膜内活菌比例明显下降。结论:金银花醇提液对粪肠球菌生物膜具有一定抑制作用。

关 键 词:粪肠球菌  金银花  生物膜

Antibacterial effect of honeysuckle ethanol extracts on enterococcus faecalis biofilm in root canal: An in vitro study
XU Ying,LI De-chao,SUN Jian.Antibacterial effect of honeysuckle ethanol extracts on enterococcus faecalis biofilm in root canal: An in vitro study[J].Heilongjiang Medicine and Pharmacy,2010,33(6):49-50.
Authors:XU Ying  LI De-chao  SUN Jian
Institution:(The College of Stomatology of Jiamusi University,Jiamusi 154002,China)
Abstract:Objective:To verify antibacterial activity of the ethanol extracts of honeysuckle against E.faecalis.Methods:The E.faecalis mono-biofilm model in vitro was established.After processed with ethanol extracts of honeysuckle,the inhibition rate of ethanol extracts of honeysuckle to E.faecalis mono-biofilm was calculated,and MTT assay was used.The effect of drugs on the E.faecalis mono-biofilm was observed by using CLSM.Results:Inhibitory effect of ethanol extracts of honeysuckle to E.faecalis mono-biofilm increased with the drug concentration.The results showed ethanol extracts of honeysuckle could decrease the vitality of E faecalis in the biofilm significantly by using CLSM.Conclusion:The effect of ethanol extracts of honeysuckle is obvious to inhibit E.faecalis mono-biofilm.
Keywords:E  faecalis  honeysuckle  biofilm
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