| 马桑内酯对瞬时外向钾电流的影响及在致痫机制中的作用 |
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| 引用本文： | 杨烨,邬丽莎,吴民泸,何涛,曾晓荣.马桑内酯对瞬时外向钾电流的影响及在致痫机制中的作用[J].山西医药杂志,2005,34(10):829-831. |
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| 作者姓名： | 杨烨 邬丽莎 吴民泸 何涛 曾晓荣 |
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| 作者单位： | 泸州医学院基础医学院，泸州医学院基础医学院，泸州医学院基础医学院，泸州医学院基础医学院，泸州医学院心肌电研究室 646000，646000，646000，646000 |
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| 摘 要： | 目的本实验拟采用全细胞膜片钳记录技术,研究马桑内酯(CL)对培养大鼠皮层神经元瞬时外向钾电流(ⅠA)的影响,从而探讨CL致癫痫的电生理机制及与ⅠA的关系,为临床更有效地防治癫痫提供新的依据。方法采用全细胞膜片钳技术记录ⅠA,进行以下方面实验①培养大鼠皮层神经元ⅠA的特性及鉴定;②三种不同浓度CL对神经元ⅠA的影响;③CL对神经元ⅠA的I-V曲线的影响。结果测试电压为+65mV时,三种不同浓度CL后均可抑制IA的电流振幅,其抑制差异均有统计学意义。结论CL具有抑制瞬时外向钾电流的作用,且在一定范围内抑制呈剂量依赖性,推测CL致癫痫的发生机制可能与IA的抑制有关。
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| 关 键 词： | 瞬时外向钾电流 癫痫 大鼠 膜片钳术 |
| 收稿时间： | 2005-06-08 |
| 修稿时间： | 2005年6月8日 |
Coriaria lactone to influence transient outward potassium current and its effect in epilepsy-induction mechanism |
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| YANG Ye, WU Li-sha, WU Min-lu ,HE Tao,ZENG Xiao-rong..Coriaria lactone to influence transient outward potassium current and its effect in epilepsy-induction mechanism[J].Shanxi Medical Journal,2005,34(10):829-831. |
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| Authors: | YANG Ye WU Li-sha WU Min-lu HE Tao ZENG Xiao-rong |
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| Institution: | Biochemistry Department of the School of Preclinical Medicine, Luzhou Medical College , Sichuan 646000, China |
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| Abstract: | Objective The objective is to observe the effect of coriaria lactone (CL) on the cortex neuron I_A of cultured newborn rat,so as to find out the electrophysiological mechanism of CL-induced epilepsy and offer a new experimental basis for preventing and treating epilepsy.Methods I_A was elicited by application of voltage-clamp steps from holding potential of-65 mV to 95 mV.I_A was respectively tested after using CL in three concentrations of 5,50,100 mg/L.Results The patch clamp experimental results demonstrated that I_A was decreased by 5,50 or 100 mg/L CL.Conclusion The results indicate that CL has an action of decreasing I_A;and the effect may be related to CL-induced epilepsy. |
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| Keywords: | Transient outward potassium current Epilepsy Rat Patch clamp techniques |
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