| TSA诱导人肝癌HepG2细胞凋亡及对FHIT表达的影响 |
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| 引用本文： | 刘晓燕,王丽,郑勇,何涛,段承刚.TSA诱导人肝癌HepG2细胞凋亡及对FHIT表达的影响[J].现代预防医学,2011,38(8):1497-1499. |
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| 作者姓名： | 刘晓燕 王丽 郑勇 何涛 段承刚 |
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| 作者单位： | 1. 泸州医学院医学分子生物学实验室,泸州,646000
2. 泸州医学院显微镜室 |
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| 摘 要： | 目的]研究去乙酰化转移酶抑制剂TSA对人肝癌HepG2细胞的作用及其FHIT表达的影响。方法]培养的人肝癌HepG2细胞随机分为两组:对照组给予等量DMSO,实验组给予终浓度分别为125、250、500、1 000、2 000nmol/L的TSA,培养24 h后收集细胞,MTT比色法检测细胞活性,TUNEL法检测细胞凋亡率,逆转录聚合酶链反应(RT-PCR)和免疫细胞化学检测FHIT的mRNA和蛋白表达水平。结果]与对照组相比,经TSA处理的细胞增殖速度明显减慢,TUNEL阳性细胞百分率随TSA浓度的升高呈剂量依赖性增高(P﹤0.01),细胞FHIT mRNA表达增强(P﹤0.01),FHIT蛋白表达差异具有统计学意义(P﹤0.01)。结论]TSA可能通过抑制HDACs的活性,上调FHIT表达,诱导细胞凋亡而抑制肝癌细胞生长。
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| 关 键 词： | 脆性组氨酸三联体 TSA 肝癌 凋亡 |
EFFECT OF TSA ON THE CELL APOPTOSIS IN THE HUMAN HEPATOMA CELL LINE HEPG2 AND THE EXPRESSION OF FRAGILE HISTIDINE TRIAD |
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| LIU Xiao-yan,WANG Li,ZHENG Yong,et al..EFFECT OF TSA ON THE CELL APOPTOSIS IN THE HUMAN HEPATOMA CELL LINE HEPG2 AND THE EXPRESSION OF FRAGILE HISTIDINE TRIAD[J].Modern Preventive Medicine,2011,38(8):1497-1499. |
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| Authors: | LIU Xiao-yan WANG Li ZHENG Yong |
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| Institution: | LIU Xiao-yan,WANG Li,ZHENG Yong,et al.(Laboratory of Medical Molecular Biology&Center of Microscope,Luzhou Medical College,Luzhou 646000,China) |
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| Abstract: | Objective]To study the role of histone deacetylase inhibitors(TSA)to the human hepatoma cell line HepG2 and the effect of fragile histidine triad(FHIT)expression.Methods]Cultured cells were divided into control group and experimental group at random,which given with DMSO and TSA(125,250,500,1 000 and 2 000 nmol/L)respectively.24 hours later the cells were collected.The effect of TSA on the activity of cells was observed by MTT.Apoptosis index was determined by using TUNEL technique and the expression of F... |
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| Keywords: | TSA |
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