| 应用PFGE及SPA-typing基因分型技术对金葡菌食物中毒溯源分析 |
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| 引用本文： | 张红宇,王冰,闫笑梅,陶晓霞,张建中,扈庆华,阚飚.应用PFGE及SPA-typing基因分型技术对金葡菌食物中毒溯源分析[J].职业与健康,2011,27(7):727-729. |
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| 作者姓名： | 张红宇 王冰 闫笑梅 陶晓霞 张建中 扈庆华 阚飚 |
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| 作者单位： | 1. 广东省深圳市疾病预防控制中心微生物检验科,518055
2. 中国疾病预防控制中心传染病预防控制所 |
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| 基金项目： | 国家科技"十一五"重大专项，深圳市科技局立项项目 |
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| 摘 要： | 目的建立金黄色葡萄球菌（金葡菌）的脉冲场凝胶电泳（PFGE）和SPA基因序列分析技术（SPA-typing）,应用于金葡菌引起的食物中毒准确溯源。方法按照GB/T4789-2003对样品进行菌株分离和生化鉴定。对同一起食物中毒分离的金葡菌株,分别用PFGE和SPA-typing技术进行分析。结果在食物中毒调查中共7份样品分离出金葡菌。该批菌株经PFGE分型呈现为一致的带型,相似性系数为100%。SPA-typing分型均为t 5593型。结论 PFGE和SPA-typing均显示患者、剩余食品和加工环境来源菌株均为同一克隆群。这是一起由于加工环境污染金葡菌引起的食物中毒。PFGE和SPA-typing分型技术均显示了良好的分型效果。
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| 关 键 词： | 金黄色葡萄球菌 脉冲场凝胶电泳 基因分型 |
Source Analysis of Staphylococcus Aureus Induced Food Poisoning by PFGE and SPA-typing Genotyping |
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| ZHANG Hong-yu,WANG Bing,YAN Xiao-mei,TAO Xiao-xia,ZHANG Jian-zhong,HU Qing-hua,KAN Biao.Source Analysis of Staphylococcus Aureus Induced Food Poisoning by PFGE and SPA-typing Genotyping[J].Occupation and Health,2011,27(7):727-729. |
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| Authors: | ZHANG Hong-yu WANG Bing YAN Xiao-mei TAO Xiao-xia ZHANG Jian-zhong HU Qing-hua KAN Biao |
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| Institution: | ZHANG Hong-yu,WANG Bing,YAN Xiao-mei,TAO Xiao-xia,ZHANG Jian-zhong,HU Qing-hua,KAN Biao(Shenzhen Center for Disease Control and Prevention,Guangdong,518055,China) |
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| Abstract: | Objective]To establish the method for source analysis of Staphylococcus aureus induced food poisoning by pulsed-field gel electrophoresis(PFGE)and SPA-typing genotyping.Methods]The isolation and biochemical identification of strains were based on the GB/T 4789-2003.The isolations from the same incident of food poisoning were analyzed respectively by PFGE and SPA-typing.Results]Staphylococcus aureus was isolated from 7 samples.The isolations had same bands confirmed by PFGE typing,the similarity coefficie... |
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| Keywords: | Staphylococcus aureus Pulsed-field gel electrophoresis(PFGE) Genotyping |
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