分化相关基因1在Ⅰ型子宫内膜癌中的表达 |
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引用本文: | 李淑霞,陈嘉薇,杨兆瑞,路光中,唐华美,胡宏慧.分化相关基因1在Ⅰ型子宫内膜癌中的表达[J].肿瘤学杂志,2008,14(3):213-215. |
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作者姓名: | 李淑霞 陈嘉薇 杨兆瑞 路光中 唐华美 胡宏慧 |
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作者单位: | 上海交通大学附属上海市第一人民医院,上海,200080 |
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摘 要: | 目的]检测NDRG1基因在正常子宫内膜和Ⅰ型子宫内膜癌中的表达,探讨其在Ⅰ型子宫内膜癌发生中的作用机制。方法]应用免疫组化、原位杂交及RT—PCR技术检测NDRG1基因在正常子宫内膜与Ⅰ型子宫内膜癌中的表达。结果]免疫组化结果显示,NDRG1蛋自在正常子宫内膜和Ⅰ型子宫内膜癌中的表达率分别为12.5%和83.5%,有显著性差异(P=0.000);原位杂交结果显示.NDRG1 mRNA在10例正常子宫内膜中只有2例表达.而在30例Ⅰ型子宫内膜癌中有22例表达,有显著性差异(P=0.007);RT-PCR结果显示,NDRG1mRNA在Ⅰ型子宫内膜癌中的表达比正常内膜增高3倍(P=0.000)。结论]NDRG1在子宫内膜癌中在蛋白水平和mRNA水平与正常子宫内膜相比都明显升高。它可能是一种肿瘤相关基因,其异常表达可能参与了Ⅰ型子宫内膜癌的发病机制。
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关 键 词: | 子宫内膜肿瘤 基因 NDRGⅠ 免疫组织化学 原位杂交 反转录聚合酶链反应 |
文章编号: | 1671-170X(2008)03-0213-03 |
修稿时间: | 2007年9月4日 |
Expression of NDRG1 in Endometrioid Adenocarcinoma Type Ⅰ |
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Institution: | LI Shu-xia, CHEN Jia-wei, YANG Zhao-rui, et al. (Shanghai First People's Hospital, Shanghai Jiaotong University, Shanghai 200080, China) |
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Abstract: | Purpose] To explore the expression of NDRG1 in normal endometrium and endometriold adenocarcinoma type Ⅰ , and to investigate its carcinogenetic mechanism in endometrioid adenocarcinoma, Methods] The expression of NDRG1 in normal endometrium and endometrioid adenocarcinoma were examined by immunohistochemistry, in situ hybridization and RT-PCR methods. Results] The immunohistochemistry result indicated that the positive expression rate of NDRG1 protein in normal endometrium and endometrioid adenocareinoma was 12.5% and 83.5% respectively, with significant difference (P=0.000); NDRG1 mRNA expressed in 2 of 10 cases of normal endometrium and 22 of 30 cases with endometrioid adenocarcinoma by in situ hybridization (P= 0.007); In RT-PCR test, NDRG1 mRNA expression was 3 times higher in endometrioid adenocarcinoma than that of normal endometrium (P=0.000). Conclusion] NDRG1 expression is upregulated significantly in endometrioid adenocarcinoma in contrast with normal endometrium both at protein and mRNA level (P〈0.01), NDRG1 might be a tumor related gene. Its overexpression may participate in the carcinogenesis of endometrioid adenocarcinoma. |
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