丁胺卡那霉素对抗凝剂依赖性血小板假性减少症血小板的解离作用及血小板功能的影响

目的研究丁胺卡那霉素对抗凝剂依赖的假性血小板减少症血小板聚集的解离作用及其功能的影响。方法①在确诊为抗凝剂依赖的假性血小板减少症患者的EDTA-K2抗凝血和枸橼酸钠抗凝血内加入丁胺卡那霉素(6.5mg/ml血),在不同时间段作血小板计数,以原液(不加丁胺卡那霉素的抗凝血)作对照。②观察血小板聚集释放的情况:在不加丁胺卡那霉素、抽血后即加丁胺卡那霉素和1小时后加丁胺卡那霉素,用胶原、ADP、花生四烯酸诱导血小板聚集释放。结果用EDTA-K2、枸橼酸钠抗凝的血小板计数随着时间的延长呈下降趋势,观察血片均有血小板聚集现象,在上述两种抗凝剂中加入丁胺卡那霉素(6.5mg/ml血),血小板计数2小时内稳定;加入丁胺卡那霉素的血小板聚集释放试验比不加丁胺卡那霉素的聚集释放试验的结果稍高。结论丁胺卡那霉素可以抑制和解离由于EDTA-K2、枸橼酸钠依赖引起的血小板聚集,对血小板的聚集释放没有影响。

Amikacin on Platelet-dependent Anticoagulant Treatment to Reduce the False Platelet Function

Objective To study the amikacin anticoagulant dependence on a variety of false thrombocytopenia platelet aggregation of dissociation, to explore the mechanism of dissociation. Methods ① Confirmed in one as a result of reliance on false anticoagulant thrombocytopenia in patients with anticoagulant EDTA and sodium citrate anticoagulation add amikacin (6.5 mg/ml blood) at different time detection of platelet in order to make a control solution (not-Jiading kanamycin anti-clotting). ②Observed in patients with non-platelet-Jiading kanamycin, after the blood-Jiading kanamycin and 1 hour after the Jiading-kanamycin, collagen, ADP, arachidonic acid induced platelet aggregation release. Results 1 : 9 in patients with anticoagulant sodium citrate appear together, 1 hour after the blood-Jiading kanamycin after the number of platelet solution and there was no significant difference. The aggregation of platelets in patients was with the release of higher test results than in the reference. Conclusion As the platelet-dependent anticoagulant on a variety of pseudo-disease reduction in platelet function reference, amikacin may be to strengthen the response to the release of platelet aggregation.