| 耐紫杉醇人肺腺癌A549细胞株中BRCA1基因启动子CpG岛甲基化的研究 |
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| 引用本文： | 尹红英,王红兵. 耐紫杉醇人肺腺癌A549细胞株中BRCA1基因启动子CpG岛甲基化的研究[J]. 实用癌症杂志, 2012, 27(4): 337-338 |
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| 作者姓名： | 尹红英 王红兵 |
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| 作者单位： | 1. 徐州医学院,221002
2. 徐州医学院附属医院,221002 |
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| 摘 要： | 目的检测耐紫杉醇人肺腺癌A549细胞株(A549/Taxol)中BRCA1基因启动子CpG岛甲基化状态,探讨A549/Taxol细胞对紫杉醇的耐药机制。方法应用甲基化特异性聚合酶链反应(MSP)技术,检测耐紫杉醇人肺腺癌A549细胞株BRCA1基因启动子CpG岛甲基化状态。结果 A549/Taxol细胞存在BRCA1基因异常甲基化,呈部分甲基化。结论 A549/Taxol细胞存在BRCA1基因异常甲基化,可能是A549/Taxol细胞对紫杉醇耐药的机制之一。
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| 关 键 词： | BRCA1基因 CpG岛 甲基化 甲基化特异性聚合酶链反应 |
The Study of CpG Island Methylation of BRCA1 Gene Promoter in a Taxol Induced Drug-resistant Human Lung Aadenocarcinoma Cell Line A549 |
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| YIN Hong-ying , WANG Hong-bing. The Study of CpG Island Methylation of BRCA1 Gene Promoter in a Taxol Induced Drug-resistant Human Lung Aadenocarcinoma Cell Line A549[J]. The Practical Journal of Cancer, 2012, 27(4): 337-338 |
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| Authors: | YIN Hong-ying WANG Hong-bing |
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| Affiliation: | .(Xuzhou Medical College,Xuzhou,221002) |
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| Abstract: | Objective To detect the CpG island methylation status of BRCA1 gene promoter in the Taxol induced drug-resistant human lung adenocarcinoma cell line A549（A549/Taxol）,and to explore the resistance mechanisms of A549/Taxol.Methods A549/Taxol were examined CpG island methylation of BRCA1 gene promoter by methylation specific PCR（MSP）.Results IBRCA1 gene aberrant methylation of A549/Taxol cells is part of methylation.Conclusion BRCA1 gene aberrant methylation of A549/Taxol may be one of the resistance mechanisms of taxol in A549/Taxol. |
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| Keywords: | BRCA1 gene CpG islands Methylation Methylation specific PCR |
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