Possible activation of murine T lymphocyte through CD98 is independent of interleukin 2/interleukin 2 receptor system |
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| Authors: | Komada Hiroshi Imai Akiyo Hattori Emi Ito Morihiro Tsumura Hideki Onoda Toshiko Kuramochi Mariko Tani Machiko Yamamoto Kanako Yamane Mizuho Kawano Mitsuo Nishio Machiko Yuasa Kimitaka O'Brien Myles Yamamoto Hidetaka Uematsu Jun Tsurudome Masato Ito Yasuhiko |
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| Affiliation: | Department of Microbiology, Mie University School of Medicine, Mie, Japan. |
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| Abstract: | CD98 is a widely expressed cell surface heterodimeric protein of 125 kDa. Its expression is upregulated during lymphocyte activation induced by mitogen, superantigen, conventional antigen, and a combination of phorbol myristate acetate (PMA) and ionomycin. However, the role of CD98 in the immune system is not so well understood. The role of CD98 in murine T lymphocyte proliferation was investigated, especially in correlation with the interleukin 2 (IL-2)/interleukin 2 receptor (IL-2R) system. Monoclonal antibody (mAb) directed against murine CD98 heavy chain (mCD98HC) suppressed the proliferation of lymphocytes stimulated with concanavalin A (Con A). Anti-mCD98HC mAb did not suppress the expression of IL-2Ralpha. Anti-IL-2Ralpha mAb, which suppressed DNA synthesis, did not inhibit the expression of CD98HC. Murine IL-2 (recombinant), which induced considerable DNA synthesis by lymphocytes stimulated with a sub-optimal dose of Con A, did not induce CD98HC expression in lymphocytes. In addition, anti-mCD98HC mAb did not inhibit the production of IL-2 by lymphocyte stimulated with Con A. Taken together with these findings, it was speculated that the CD98 system is independent of the IL-2/IL-2R system in murine T lymphocyte activation. |
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