两种IgA肾病大鼠模型的比较

目的 探讨快速建立IgA肾病大鼠模型的方法.方法 雌性SD大鼠被随机分为3组.A组采用口服牛血清白蛋白(BSA) 葡萄球菌肠毒素B(SEB)方法,B组采用口服BSA SEB 皮下注射CCl4方法,C组为正常对照组.分别于10、14周处死后取动脉血、肾脏,检测血生化指标,检查组织病理及lgA免疫荧光.于第10、12、14周收集24h尿检测尿蛋白.结果 与空白对照组相比,A组和B组血肌酐、尿素氮和尿蛋白均显著增高(P<0.05),有中度肾小球系膜扩张伴系膜细胞增生,A组和B组之间无明显差异(P>0.05).但B组出现血尿、蛋白尿早于A组,且IgA免疫荧光强于B组.结论 口服BSA SEB法与口服BSA SEB 皮下注射CCl4方法均能诱发SD大鼠IgA肾病模型,后者更早出现血尿、蛋白尿,肾脏IgA免疫荧光更强,是更为省时的制模方法.

Comparison of two rat models of IgA nephropathy

Objective To study the methods for rapid establishment of rat models of IgA nephropathy. Methods Forty female SD rats weighing 160-200 g were randomized into 3 groups. In group A, the rats received intravenous injection of staphylococcal enterotoxin B (SEB) and oral bovine serum albumin (BSA), and in group B, CCl4 was injected subcutaneously in addition to the above treatments; the rats in group C received no treatments to serve as the normal control group. The rats were sacrificed 10 and 14 weeks after the...