软骨源形态发生蛋白基因转染自体骨髓间充质细胞修复关节软骨缺损

背景：以支架或干细胞在一定程度上能够修复软骨缺损，但是研究中发现较大块的缺损修复效果还达不到令人满意的程度。基因转染后的干细胞能够不断的释放生长因子有可能够为软骨缺损研究带来突破。 目的：进一步验证软骨源形态发生蛋白转染的骨髓间充质干细胞是否会促进体内兔软骨修复。 方法：从兔骨髓内分离获得骨髓间充质干细胞，脂质体感染的方法将软骨源形态发生蛋白基因转染到骨髓间充质干细胞中。实验组移植转染后的细胞；单纯骨髓间充质干细胞组移植未转染的骨髓间充质干细胞；空白对照组缺损区不移植细胞。术后行组织学检测及组织学评分分析修复情况。 结果与结论：体内修复过程中，软骨源形态发生蛋白转染的骨髓间充质干细胞促进了软骨再生。透明软骨填充了软骨缺损区，并且深部区域显示了软骨下成骨。透明软骨的重建区域优于单纯骨髓间充质干细胞移植组。组织学评分实验组高于骨髓间充质干细胞对照组和空白组。提示转染软骨源形态发生蛋白基因的骨髓间充质干细胞能够促进和提高关节软骨的改建和修复。

Repairing articular cartilage defects with cartilage-derived morphogenetic protein transfected autologous bone marrow mesenchymal stem cells

BACKGROUND: It can repairing cartilage defect somehow with scarffolds or stem cells, but we found that the effect of repair were not satisfied in large defects. The gene transfected stem cells can continuously release grow factors, and this may be presented a progress in cartilage defect. OBJECTIVE: To verify whether cartilage-derived morphogenetic protein 1 (CDMP1)-transfected autologous bone marrow mesenchymal stem cells (BMSCs) enhance cartilage repair in rabbit in vivo. METHODS: BMSCs were isolated from bone marrow of rabbits, and transfected with the CDMP1 gene by the lipofection method. The autologous cells were then implanted into full-thickness articular cartilage defects in the knee joints of rabbit as experimental group; BMSCs were transplanted as control BMSCs group and no cells transplanted as blank control group. The repair was analyzed by histological detection and histological score following surgery. RESULTS AND CONCLUSION: During in vivo repair of articular cartilage defects, cartilage regeneration was enhanced by the implantation of CDMP1 transfected autologous BMSCs. The defects were filled by hyaline cartilage and the deeper zone showed remodeling to subchondral bone overtime. There pair and reconstitution of zones of hyaline articular cartilage was superior to simple BMSCS implantation. The histological score of the CDMP1 transfected BMSCs group was significantly better than those of the BMSCs control group and the blank control group. These indicate that CDMP1 transfected BMSCs enhanced repair and remodeling of articular cartilage.