Detection of c-Ki- ras point mutation from pancreatic juice |
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Authors: | Hiroshi Miki Shuji Matsumoto Hideo Harada Seiji Mori Reiji Haba Koji Ochi Shoji Kobayashi and Masaki Ohmori |
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Institution: | (1) Department of Pathology, Kagawa Medical School, 1750-1, Miki, Kita, 761-07 Kagawa, Japan;(2) Department of Laboratory Medicine, Okayama University Medical School, Okayama, Japan |
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Abstract: | Summary Cytological diagnosis of pancreatic carcinoma sometimes poses difficulties in distinguishing malignant from benign cells.
Recent molecular study of pancreatic carcinoma has revealed a very high incidence of a point mutation of the c-Ki-ras oncogene at codon 12 in this neoplasm. To take advantage of this technique for the diagnosis of pancreatic carcinoma, we
attempted to amplify the c-Ki-ras gene from endoscopically obtained pancreatic juice by isolation of DNA and polymerase chain reaction (PCR) coupled with restriction
fragment length polymorphism (RFLP). PCR was possible in approx 70% of the cases. A point mutation was nonradioisotopically
detected in 4 of 6 pancreatic carcinomas and in one intraductal papillary neoplasm, whereas no mutation was detected in other
cases. Thus, this method was thought to be useful for the diagnosis of pancreatic carcinoma. |
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Keywords: | Pancreatic carcinoma K-ras point mutation pancreatic juice PCR RFLP |
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