血清 miR-141和 miR-143联合检测非小细胞性肺癌的诊断价值

目的：探讨血清中 microRNA 作为非小细胞性肺癌生物标志物的诊断价值。方法采用定量 RT-PCR 检测训练组中30例非小细胞性肺癌患者血清、30例健康对照者血清中差异表达的 microRNA，采用受试者工作曲线和曲线下面积来筛选作为候选标志物的 microRNA，建立诊断模型，再用15例非小细胞肺癌和15例健康对照者中的候选 miRNA 的表达情况去验证模型（验证组）的诊断效能，计算灵敏度、特异度、阳性预测值、阴性预测值等诊断价值。结果训练组中 miR-141和 miR-143表达与对照组比较差异有统计学意义（P ＜0．05），在验证组中二者联合的诊断效能分别为：灵敏度为99％（95％CI ：0．94～1．0），特异度为85％（95％CI ：0．75～0．93），阳性预测值为87％，阴性预测值为99％。结论血清 miR-141和 miR-143是非小细胞性肺癌潜在的诊断标志物。

Serum miR-141 and miR-143 as biomarkers for detection of non-small cell lung cancer

Objective To evaluate the significance of serum miR-141 and miR-143 level as early biomarkers for non small cell lung cancer (NSCLC).Methods Serum miR-141 and miR-143 level were measured by qRT-PCR.90 samples were divided into two sets,training set and the test set.In training set,it was compared in two groups of:30 patients with NSCLC,30 healthy volunteers and screened some candidate microRNA differential pairs(miR-141 and miR-143).In the test set (15 patients with NSCLC,15 healthy volunteers)we validated the value of diagnostic with miR-141 and miR-143 and calculated some data:sensitivity,specificity, positive predictive value(PPV)and negative predictive value(NPV).Results In training set,the expression of miR-141 and miR-143 were significant different compared to the control group.In the test set,the diagnostic values of miR-141 and miR-143 were as follow:sensitivity 99%(95%CI :0.94-1.0),specificity 85% (95%CI :0.75-0.93),PPV87% and NPV 99%.Conclusion Serum miR-141 and miR-143 are promising early biomarkers for NSCLC.