唇癌微血管密度和血管内皮生长因子表达的意义

目的　研究唇鳞状细胞癌组织中微血管密度 (MVD)和血管内皮生长因子 (VEGF)的表达和意义。方法　采用CD34和VEGF单克隆抗体 ,用免疫组化S P法对 4 2例唇鳞状细胞癌标本进行免疫组化染色。结果　唇癌组织MVD显著高于正常对照唇组织MVD(P <0 .0 0 1)。有淋巴结转移唇癌的MVD高于无淋巴结转移的MVD ,但两者之间无显著性差异 (P >0 .0 5 )。唇癌组织和正常组织中VEGF表达阳性率分别为 6 1.90 %和 10 .0 0 % ,两者间有显著性差异 (P <0 .0 0 1)。有淋巴结转移组VEGF阳性率 (80 .0 0 % )高于无淋巴结转移组VEGF阳性率 (5 6 .2 5 % ) ,但两者间无显著性差异 (P >0 .2 5 )。VEGF阳性表达唇癌的MVD高于VEGF阴性表达唇癌的MVD ,两者间有显著性差异 (P <0 .0 2 )。结论　唇癌中VEGF的表达与MVD有密切的联系 ,说明VEGF能较好地反映唇癌微血管生成的活跃程度 ,是一个重要促血管生成因子

Microvessel Density and Expression of Vascular Endothelial Growth Factor in Squamous Cell Carcinomas of Lip

Objective To determine the expression of vascular endothelial growth factor (VEGF) and the effects of angiogenesis on cervical lymph node metastasis and progression of squamous cell carcinomas (SCC) of lip. Methods 42 samples with SCC of lip were stained using the anti-CD34 and anti-VEGF monoclonal antibody by immunohistochemical S-P method to determine blood vessels and VEGF Expression. Results The MVD of the tumor was significantly higher than that of normal control tissue(P<0.001), and the positive rate (61.90%) of VEGF expression in tumors was significantly higher than that in normal control tissues(10.00%, P<0.001). The MVD of the VEGF positive cases in SCC of lip was significantly higher than that of the VEGF negative cases(P<0.05), while the difference of MVD and VEGF expression had no significance between the cases with and without lymph node metastasis (P>0.05). Conclusion There is an association between angiogenesis and tumor progression, and the expression of VEGF is much related to MVD in SCC of lip.