糖尿病视网膜病变血浆与眼内液血管内皮生长因子的相关研究

目的 通过检测糖尿病性视网膜病变(DR)患者血浆、眼内液(房水、玻璃体)血管内皮生长因子(VEGF)含量，探讨其在DR发展变化中 的作用。 方法 采用双抗体夹心酶联免疫吸附试验ELISA法定量检测患者组及对照组血浆、房水、玻璃体VEGF含量。 结果 无糖尿病性视网膜病变(NDR)组血浆VEGF含量(34.47±1.76) pg/ml ，单纯型糖尿病性视网膜病变(BDR)组血浆VEGF含量(53.93±3.08) pg/ml，增生型糖尿病性视网膜病变(PDR)组血浆VEGF含量(53.36±3.28) pg/ml，对照组血浆VEGF含量(178.30±10.13) pg/ml，与实验组比较差异均有显著性的意义(P＜0.05)；PDR组房水VEGF含量(184.86±1260) pg/ml，对照组房水VEGF含量(90.06±18.32) pg/ml，两者比较差异有显著性的意义(P＜0.05)；PDR组玻璃体VEGF含量(741.70±92.02) pg/ml，对照组玻璃体VEGF含量(94.38±21.21) pg/ml，两者比较差异有显著性的意义(P＜0.05)。PDR组血浆VEGF与房水、玻璃体VEGF无相关关系(P＞0.05)，玻璃体VEGF与糖化血红蛋白(HbA1c)有正相关关系(r=0.9067,P＜0.01)。 结论 糖尿病患者血浆VEGF含量较正常人低，但与房水、玻璃体VEGF含量无关；增生型糖尿病性视网膜病变患者房水与玻璃体VEGF含量增高，玻璃体内VEGF含量增高与患者HbA1c值有关。 (中华眼底病杂志，2004，20：343-345)

Investigation of concentration of vascular endothelial growth factor in plasma and intraocular liquid in diabetic retinopathy

Objective To observe the clinical manifestations and fe at ures of fundus fluorescein angiograms (FFA) of patients with multifocal choroidi tis (MFC). Methods The data of 8 patients who had been diagnos ed by clinic and FFA as with MFC were collected, and their clinical manifestatio ns and results of FFA were analyzed and valued. Results The ag e of the 7 female and 1 male patients ranged from 16 to 32, and all of the 8 pa tients had high myopia (from -6.00 D to -8.00 D) with binocular multiple small yellowish white lesions in posterior pole of the fundus and a few phlogistic ce lls in vitreous body. In addition, macular choroidal neovascularization membrane (CNV) was found in 6 patients, binocular in 2 and monocular in 4. The results o f FFA in 7 patients showed hypofluorescence of the yellowish-white lesions at t he early phase and pigmentation at the late phase; Corresponding manifestations of FFA could be found in the patients attended by CNV surrounded by leakage. Conclusion MFC are mostly diagnosed in young females with myopia. Most of the patients had binocular affection with multiple small yellowish whit e lesions at the posterior pole, whose FFA shows hypofluoresence of the active l esions at the early phase and pigmentation at the late phase. CNV may occur in p atients with MFC.