Foxp3,TGF-β1,VEGF,CD3-ζ和IL-10在卵巢上皮性癌中的表达与预后相关分析

 目的 研究叉头蛋白 3（Foxp3）、转化生长因子 β1（TGF- β1）、血管内皮生长因子（VEGF）、CD3-ζ、IL-10 等免疫抑制因子的表达强度与卵巢上皮性癌预后的相关性。 方法 采用免疫组织化学 SP 法，检测 5 种因子分别在79 例卵巢上皮性癌、16 例卵巢交界性肿瘤、30 例卵巢良性肿瘤和 20 例正常卵巢组织中的表达情况，应用计算机图像分析系统和人工半定量评分方法分别对 Foxp3、TGF-β1 和 VEGF、CD3-ζ、IL-10 的表达结果进行评判，并分析 5 种因子在卵巢上皮性癌中表达强度相互之间的相关性，及其表达强度与卵巢上皮性癌预后的相关性。 结果 TGF-β1 在正常卵巢组织中的表达强度明显低于卵巢上皮性癌、卵巢交界性肿瘤和卵巢良性肿瘤组织（P = 0.009），而其他 4 种因子在卵巢上皮性癌组织中的表达强度均明显高于卵巢交界性肿瘤、卵巢良性肿瘤和正常卵巢组织（均 P < 0.001）。TGF-β1 的表达强度只与 CD3-ζ 有关（P = 0.001），CD3-ζ 的表达强度与其他 4 种因子均有关（P < 0.01），Foxp3、VEGF、IL-10 的表达强度相互之间均具有明显的相关性（均 P < 0.01）。Foxp3 和 TGF-β1 的表达强度均与卵巢上皮性癌的总生存期有关（P 值分别为 0.010、0.013），而 VEGF、CD3-ζ、IL-10 的表达强度均与卵巢上皮性癌的总生存期无相关性；Logistic 回归分析显示，TGF-β1、术后残余和化疗规范性为卵巢上皮癌的 3 个重要预后因素（P < 0.05）；在卵巢上皮性癌中，TGF-β1 的表达强度与患者年龄、FIGO 分期、病理分级、术后残余、淋巴结转移以及化疗规范性等病理特征均无相关性。 结论 5 种免疫抑制因子在卵巢上皮性癌组织中均呈高表达，Foxp3 和 TGF-β1 的表达强度与卵巢上皮性癌的预后有关。

Relationship between expression of Foxp3, TGF-β1, VEGF, CD3-ζ, and IL-10 in ovarian cancer tissues and the prognosis of the disease

Objective To investigate the correlation between the expression of immunosuppressors, including Foxp3, TGF-β1, VEGF, CD3-ζ, and IL-10, and prognosis of epithelial ovarian cancer. Methods A total of 79 specimens of epithelial ovarian cancer, 16 cases of borderline ovarian tumor, 30 cases of benign ovarian tumor, and 20 cases of normal ovarian tissue were used in this study. The expression of Foxp3, TGF-β1, VEGF, CD3-ζ, and IL-10 were detected by SP method, and then analyzed by standard pathologic review and semi-quantitative imaging. The relationship among the levels of expression of the 5 immunosuppressors, as well as the correlation between the expressions and the prognosis of epithelial epithelial ovarian cancer were then evaluated. Results The expression level of TGF-β1 in normal ovarian tissue was significantly lower than that in epithelial, borderline, and benign ovarian tumors (P=0.009); whereas the levels of the expression of Foxp3, VEGF, CD3-ζ, and IL-10 in epithelial ovarian cancer were significantly higher than those in the borderline, and benign tumors, as well as the normal tissues (all P < 0.01). Correlation among the expression levels of Foxp3, VEGF, and IL-10 were observed (all P < 0.01). The total survival rate was related to the expression levels of Foxp3 and TGF-β1 (P = 0.010 and 0.013 respectively), but not with that of the VEGF, CD3-ζ, and IL-10. Logistic regression revealed that the expression of TGF-β1, residual tumor, and chemotherapy standard were 3 important prognostic factors of epithelial ovarian cancer. In patients with epithelial ovarian cancer, no correlation between the expression level of TGF-β1 and age, FIGO stage, TNM stage, residual tumor, lymphatic metastasis, or chemotherapy standard was observed. Conclusions The 5 immunosupressors are highly expressed in epithelial ovarian cancer. The expression levels of Foxp3 and TGF-β1 are correlated with the prognosis of the patients with the cancer.