人类胃癌裸鼠淋巴结转移模型的建立

 目的　研究胃癌淋巴结转移动物模型的建立方法。方法　人类胃癌低分化细胞系SGC-7901体外培养、传代并扩增后，收集细胞行皮下种植成瘤，鼠间传代至第6代，以皮下肿瘤组织块原位种植于裸鼠胃壁建立动物模型。种植后第9周处死裸鼠，观察原位种植瘤生长、淋巴结转移及其他脏器转移情况，测定荷瘤裸鼠血清癌胚抗原（CEA）值。结果　原位移植瘤种植成功率100 ％，胃周淋巴结转移率93.3 ％，移植瘤可发生局部浸润及远处脏器转移，荷瘤裸鼠CEA值明显高于正常裸鼠（P＜0.01）。结论　应用SGC-7901细胞系可成功建立胃癌的淋巴结转移动物模型。

Establishment of lymphatic metastasis model for human gastric cancer in nude mice

Objective　To investigate the method of establishing lymph node metastasis model for gastric cancer in nude mice. Methods　Human poorly differentiated gastric cancer SGC-7901 cells were cul－tured, inherited, amplified and collected in vitro, and were inoculated in nude mice subcutaneously. After pas－saging six generations, the subcutaneous tumor was orthotopically implanted in nude mice. The mice were then sacrificed on the 9th week after implantation. Serum CEA density was detected using radio-immunity method. Orthotopically implanted tumors, remote organ metastases as well as lymph node metastasis were observed macroscopically and histologically. Results　The successful rate of orthotopically transplantion of gastric can－cer was 100 %. The rate of perigastric lymph node metastases was 93.3 %. Local infiltration and remote metastasis were observed. The level of serum CEA in nude mice bearing tumor was significantly higher than that of normal nude mice (P <0.01). Conclusion　Animal model of gastric cancer lymph node-metastasis was successfully established using SGC-7901 cell line.