| 大鼠肝癌组织中GD_3合成酶升高的特异性研究 |
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| 引用本文： | 谢天培,夏霞娟,顾天爵.大鼠肝癌组织中GD_3合成酶升高的特异性研究[J].复旦学报(医学版),1994(4). |
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| 作者姓名： | 谢天培 夏霞娟 顾天爵 |
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| 作者单位： | 上海医科大学基础医学院生物化学教研室 |
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| 摘 要： | 作者改进了鞘糖脂糖基转移酶的检测方法，用ＳｅｐｈａｄｅｘＧ－５０Ｆｉｎｅ成功地分离了酶反应的同位素标记产物与底物。从而缩短了酶活性的测定时间，简化了操作步骤，并减少了同位素的污染机会。在此基础上系统检测了二乙基亚硝胺诱发肝癌大鼠及正常大鼠各脏器（肝、脾、肾、心、脑、肺、胃）ＧＤ３合成酶（ＣＭＰ－ＮｅｕＡｃ：ＧＭ３唾液酸转移酶；ＳＴ２）及目前报道的其他４种鞘糖脂唾液酸转移酶（ＳＴ１、３、４、５）的活性，企图从中选择一种ＳＴ２含量高、活性高的组织作为酶分离纯化的材料。结果表明，ＳＴ２在二乙基亚硝胺诱发肝癌大鼠肝癌组织中特异性升高，从而提示此组织可用作ＳＴ２分离纯化的最佳材料。
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| 关 键 词： | 肝癌，GD_3合成酶，鞘糖脂糖基转移酶 |
SPECIFIC INCREMENT OF GD_3 SYNTHETASE IN RAT LIVER CANCER |
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| Xie Tianpei, Xia Xiajuan, Gu Tianjue.SPECIFIC INCREMENT OF GD_3 SYNTHETASE IN RAT LIVER CANCER[J].Fudan University Journal of Medical Sciences,1994(4). |
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| Authors: | Xie Tianpei Xia Xiajuan Gu Tianjue |
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| Abstract: | A method utilizing Sephadex G-50 Fine column has been developed that allows the rapid separation of radiolabelled products of glycosphingolipid:Sialyltransferases (ST) from their substrate (CMP-14C-SA).Using this method,the activities of ST1-5 in several animal organs have been determined and the specific increment of ST2 activity was found in diethyl-nitrosamine induced rat hepatoma. |
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| Keywords: | liver cancer GD_3 synthetase Glycosphingolipid: Sialyltransferases |
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