bFGF 在Medpor- 肋软骨联合兔耳廓再造术

目的 观察碱性成纤维细胞生长因子（basic fibroblast growth factor，bFGF） 在Medpor- 肋软骨联合耳廓再造术中的应用效果，探讨其使用意义。 方法 构建兔肋软骨与Medpor 耳廓复合支架模型，植于兔背部皮下，将实验动物分成两组：肋软骨与Medpor 材料直接连接组（A 组） ；肋软骨与Medpor 材料直接连接后bFGF 处理组（ 术中及术后2 d，每日局部注射bFGF 200 ng）（B 组）。植入后7 d、14 d、28 d 取出复合支架，行组织学观察，免疫组织化学法（SABC 法），检测Medpor 片边缘及内部CD34 表达，并行微血管密度（microvessel density，MVD） 计数。 结果 B 组术后早期Medpor 材料周边纤维组织增生与血管化较A 组明显，但后期血管化程度与A 组无明显差异。 结论 术后早期应用bFGF 对复合支架的纤维化及血管化过程起到明显的促进作用，加强了Medpor 与软骨片的连接，增强了复合支架的稳定性及抗感染力。

bFGF in rabbit ear reconstruction with Medpor and costal cartilage

Objective To study the effect of bFGF in ear reconstruction with Medpor-costal cartilage. Methods A rabbit costal cartilage and Medpor scaffold model was established and implanted into the back skin of rabbits. The animals were divided into direct costal cartilage and Medpor connection group （group A） and bFGF-treated direct costal cartilage and Medpor connection group （group B）. The scaffold was taken out on days 7, 14 and 28 after it was implanted. Its morphology was observed by histoimmunohistochemistry. CD34 expression in Medpor was detected and its microvessel density （MVD） was measured. Results The fibrosis and vascularization in Medpor were more significant in group B than in group A early after operation with no significant difference observed between the two groups later after operation. Conclusion bFGF plays a significant role in promoting fibrosis and vascularization of the scaffold early after operation, thus strengthening its connection to Medpor and cartilage, and increasing its stability and anti-infection ability.