缺氧复氧性心肌细胞损伤过程中钙敏感受体作用探讨

目的观察钙敏感受体（CaSR）在缺氧复氧性大鼠心肌细胞损伤过程中的作用。方法将45份原代培养4d的大鼠心肌细胞按随机数字法分为正常对照组、缺氧／复氧组、钙阻断剂组、CaSR激动剂组和钙耗竭组,各9份。用饱和氮气pH6．8的D—Hands液培养心肌细胞3h,再用含20％新生牛血清的DMEM液培养心肌细胞9h,复制心肌缺氧／复氧模型。测定肌酸激酶（cK）含量。结果不同组CK含量差异有统计学意义（P〈0．01）。缺氧／复氧组、钙阻断剂组及CaSR激动剂组CK含量均高于正常组（均P〈0．05）,钙耗竭组与正常组CK含量差异无统计学意义（P〉0．05）;钙阻断剂组、CaSR激动剂组及钙耗竭组的CK含量均低于缺氧／复氧组（均P〈0．01）;CaSR激动剂组的CK含量高于钙阻断剂组和钙耗竭组（均P〈0．05）。结论CaSR参与大鼠缺氧复氧性心肌细胞损伤。

The effect of calcium-sensing receptor on myocardial hypoxia/reoxygenation injury in rats

Objective To observe the role of calcium-sensing receptor（CaSR） in myocardial hypoxia/reoxygena- tion in rats. Methods Primary cardiomyocytes were cultured for four days, then randomly divided into five groups： control group, hypoxia/reoxygenation group（H/Re）, calcium channel inhibition group, CaSR activation group and cal- cium depletion group. The cardiomyocytes were in ischemia-mimetic solution for three hours, and re-incubated in normal culture medium for nine hours to establish a model of H/Re. The levels of creatine kinase （CK） were meas- ured. Results CK level was significantly increased after hypoxia/reoxygenation. The CK level was lower in the Na＋ -Ca2＋ exchanger and L-calcium channel inhibition group compared with that in the H/Re group. CK level was signifi- cantly higher in the CaSR group than that in the calcium channel inhibition group and calcium depletion group （P 〈 0.05）.Conclusions CaSR was involved in the hypoxiaJreoxygenation injury in rats.