Dishevelled蛋白表达与脑胶质瘤增殖、侵袭的相关性研究

目的 探讨Dishevelled蛋白表达与脑胶质瘤细胞增殖、侵袭的相关性.方法 选取67例脑胶质瘤患者组织标本,采用免疫组织化学法检测Dishevelled蛋白的表达,评定Dishevelled蛋白免疫反应评分（IRS）、肿瘤增殖指数（PI）和侵袭指数（Ⅱ）,并分析其相关性.结果 67例脑胶质瘤中,Dishevelled蛋白阳性率为65.7％（44/67）,Dishevelled蛋白IRS及肿瘤PⅠ、Ⅱ分别为4.15±3.13、（30.93±17.92）％、（20.38±13.36）％,均随脑胶质瘤病理级别的增高而升高（P＜0.001）,Dishevelled蛋白阳性组PⅠ、Ⅱ高于Dishevelled蛋白阴性组（38.27±17.60）％比（16.02±8.92）％;（30.03±13.81）％比（10.63±4.41）％,均P＜ 0.001].肿瘤PⅠ、Ⅱ与Dishevelled蛋白IRS呈正相关（相关系数r分别为0.940、0.953）.结论 Dishevelled蛋白高表达在脑胶质瘤恶性增殖、侵袭中起着重要的作用.

Expression of Dishevelled protein is correlated with the proliferation and invasion of glioma

Objective To investigate the correlation between Dishevelled protein expression and the proliferation and invasion of glioma cells.Methods 67 cases of brain glioma specimens were collected.The expression of Dishevelled protein was detected with immunohistochemical method.The immunoreactivity score （IRS） of Dishevelled protein,and proliferation index （PⅠ） and invasion index （Ⅱ） were measured and their correlations were analyzed.Results The positive rate of Dishevelled protein in glioma was 65.7 % （44/67）.IRS,PⅠ and Ⅱ were 4.15±3.13,（30.93±17.92） %,（20.38±13.36） %,respectively.Both PⅠ and Ⅱ significantly increased with an increase in the pathological grade of brain glioma （P 〈 0.001）.Furthermore,PⅠ and Ⅱ were significantly higher in the Dishevelled protein-positive group than those in the Dishevelled protein-negative group （38.27±17.60） % vs （16.02±8.92） % of PⅠ and （30.03±13.81） % vs （10.63±4.41） % of Ⅱ,respectively,P 〈 0.001].PⅠ and Ⅱ of glioma cells were positively correlated with IRS of Dishevelled protein （r =0.940 between PⅠ and IRS,and r =0.953 between Ⅱ and IRS,respectively）.Conclusion Dishevelled protein plays an important role in the proliferation and invasion of brain malignant glioma.