细胞外基质蛋白Periostin、血管内皮生长因子和基质金属蛋白酶-9在乳腺浸润性导管癌中的表达及其临床意义

目的探讨细胞外基质蛋白Periostin、血管内皮生长因子（VEGF）和基质金属蛋白酶-9（MMP-9）在乳腺浸润性导管癌中的表达差异及其与临床病理特征的关系。方法应用免疫组织化学法检测58例乳腺浸润性导管癌组织和25例癌旁正常乳腺组织中Periostin、VEGF和MMP-9的表达水平。结果在乳腺浸润性导管癌和正常乳腺组织中，Periostin的阳性率分别为63．8％（37／58）和0，VEGF的阳性率分别为69．0％（40／58）和8．0％（2／25），MMP-9的阳性率分别为70．7％（41／58）和16．0％（4／25），三者在两组间阳性率差异均有统计学意义（均P〈0．001）。Periostin阳性率与TNM分期有关（X2=4．835，P=0．028），与患者年龄、组织学分级、淋巴结转移均无关；VEGF阳性率与TNM分期、淋巴结转移有关（X2=4．230，P=0．040；X2=9．667，P=0．002），与患者年龄、组织学分级无关（均P〉0．05）；MMP-9阳性率与TNM分期、淋巴结转移有关（x2=8．456，P=0．004；X2=5．494，P=0．019），与患者年龄、组织学分级无关（均P〉0．05）。Periostin、VEGF和MMP-9在乳腺浸润性导管癌组织中呈两两正相关（r分别为0．348、0．303、0．469，均P〈0．05）。结论Periostin、VEGF和MMP-9在乳腺浸润性导管癌的发生、发展及侵袭转移中起重要作用，它们在乳腺浸滑『生导管癌的表达两两相关。

Expression and clinical significance of Periostin,VEGF and MMP-9 in breast invasive ductal carcinoma

Objective To investigate the expression and significance of Periostin, VEGF and MMP-9 in breast invasive ductal carcinoma. Methods Immunohistoehemistry was employed to detect the expression of Periostin, VEGF and MMP-9 in breast invasive ductal carcinoma and normal breast tissue. Results In breast invasive ductal carcinoma and normal breast tissue ,the positive rates of Periostin were 63.8 % （37/58） and 0 （X2 = 24.272, P = 0.000）. The figures were 69 % （40/58） and 8 % （2/25） for the positive rates of VEGF （X2 = 25.977, P = 0.000）, respectively, as well as 70.69 % （41/58） and 16.0 % （4/25） for the positive rates of MMP-9 （X2 = 21.050, P = 0.000）, respectively. There were significant differences among the groups （P 〈 0.05）. In breast invasive ductal carcinoma, the expression of Periostin was correlated with clinical stage （X2 = 4.835, P = 0.028）, whereas not correlated with age （X2 = 1.155, P = 0.282）, histological grade （X2 = 0.05, P = 0.972）, lymphatic metastasis （X2 = 1.660, P = 0.198）. The expression of VEGF was correlated with clinical stage （X2 = 4.230, P = 0.040）, lymphatic metastasis （~ = 9.667, P = 0.002）, whereas not correlated with age （X2 = 0.506, P = 0.477）, histological grade （X2 = 0.532, P = 0.767）. The expression of MMP-9 was correlated with clinical stage （X2 = 8.456, P = 0.004）, lymphatic metastasis （x2= 5.494, P = 0.019）, whereas not correlated with age （x2 = 0.153, P = 0.695）, histological grade （X2 = 0.224, P = 0.894）. The expression of Periostin, VEGF and MMP-9 were positively correlated with each other in breast invasive ductal carcinoma （r = 0.348, P = 0.001; r = 0.303, P = 0.021; r = 0.469, P = 0.000）. Conclusion Periostin,VEGF and MMP-9are correlated closely with the occurrence and development of breast invasive ductal carcinoma, which might be valuable in evaluating the invasiveness, metastasis and prognosis.