| 双基质免疫荧光法检测抗核抗体在风湿病中的应用 |
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| 引用本文： | 郭雨凡,陈志伟,顾美华,邓迎苏,武剑.双基质免疫荧光法检测抗核抗体在风湿病中的应用[J].苏州大学学报(自然科学版),2005,25(4):624-626. |
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| 作者姓名： | 郭雨凡 陈志伟 顾美华 邓迎苏 武剑 |
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| 作者单位： | 苏州大学附属第一医院风湿免疫科,江苏苏州215006 |
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| 摘 要： | 目的研究双基质免疫荧光法检测抗核抗体（ANA）在风湿病中的应用价值。方法采用以喉癌上皮细胞和猴肝细胞为底物的间接免疫荧光法检测211例弥漫性结缔组织病患者（CTD）和43例其他风湿病患者的血清ANA。结果CTD中ANA的阳性率为84．1％，显著高于非CTD风湿病组的2.3％（P〈0．01）。ANA阳性者可呈现不同的荧光模式。结论ANA是区别CTD与非CTD风湿病的重要指标，不同的荧光模式有不同的意义。
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| 关 键 词： | 抗核抗体 结缔组织病 免疫荧光 |
| 文章编号： | 1673-0399(2005)04-0624-03 |
| 收稿时间： | 2004-09-01 |
| 修稿时间： | 2004年9月1日 |
The Significance of ANA Detected by Double Substrates Immunofluoresence Assay in Rheumatism |
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| GUO Yu-fan, CHEN Zhi-wei, GU Mei-hua, et al.The Significance of ANA Detected by Double Substrates Immunofluoresence Assay in Rheumatism[J].Suzhou University Journal of Medical Science,2005,25(4):624-626. |
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| Authors: | GUO Yu-fan CHEN Zhi-wei GU Mei-hua |
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| Institution: | Dept of Rheumatology, the First Hospital Affiliated to Suzhou University, Suzhou 215006, China |
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| Abstract: | Objective To study the significance of ANA detected by double substrates(immunofluorescence) assay.Methods Hep2 cells and monkey liver tissue were compared as substrates for detection of ANA in serum in 211 pations with CTD and 43 non-CTD subjects,using indirect immunofluoresence assay.Results The positive rate of ANA in CTD group was 84.1%,in non-CTD group was 2.3%,there was significant difference(P<0.01).There were different fluorescence mo-(dels of) ANA in the positive objects.Conclusion ANA detected by double substrates immunofluorescence assay is an important method to identify CTD and non-CTD.Different fluorescence models have different significance. |
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| Keywords: | ANA connected tissue disease immunofluorescence |
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