腺病毒介导ｐ１６基因表达对胃癌细胞化疗敏感性的增强作用*

目的　研究ｐ１６基因对胃癌细胞化疗敏感性的作用。方法　构建携带ｐ１６基因的腺病毒感染胃癌细胞株ＳＧＣ-７９０１；Ｗｅｓｔｅｒｎｂｌｏｔｔｉｎｇ鉴定癌细胞ｐ１６的表达；ＭＴＴ法检测顺铂（ＤＤＰ）对癌细胞存活率的影响；动物体内实验验证ｐ１６与ＤＤＰ对癌细胞生长抑制的协同作用。结果　腺病毒感染胃癌细胞后表达ｐ１６使癌细胞对ＤＤＰ更为敏感，其中ｐ１６阳性组的细胞在ＤＤＰ浓度达到１.５ｍｇ／Ｌ时，细胞存活率降至２０％以下，而同期ｐ１６阴性组的细胞在相同ＤＤＰ浓度时存活率仅下降至６０％左右。ｐ１６腺病毒联合ＤＤＰ对裸鼠移植瘤的生长抑制明显强于单独应用ｐ１６腺病毒或ＤＤＰ。结论　胃癌细胞获得外源性ｐ１６表达对ＤＤＰ的敏感性提高，ｐ１６基因治疗联合化疗将有可能提高抗肿瘤效果。

Adenovirus-mediated p16 expression enhances chemotherapeutic sensitivity of gastric cancer cells

Objective To investigate the effect of p16 gene on chemotherapeutic sensitivity of gastric cancer cells. MethodsAdenovirus-armed p16 gene was constructed and used to infect gastric cancer cell line SGC-7901.Western blotting was used to detect p16 expression,MTT assay was used to examine the viability of cancer cells after treatment with cisplatin(DDP).The antitumor effect of p16 combined with DDP was proved in xenografts in nude mice. Resultsp16 re-expressed after infection with adenovirus carrying p16 gene...